Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Mouse IgG2a (Chimeric)

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Mouse IgG2a (Chimeric) (AB319022)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh frozen) tissue labeling GFAP with ab319022 at 1/500 (1.0 ug/ml) dilution (Green).

Confocal image showing positive staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab319022 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Mouse IgG2a (Chimeric) (AB319022)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh frozen) tissue labeling GFAP with ab319022 at 1/500 (1.0 ug/ml) dilution (Green).

Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab319022 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Mouse IgG2a (Chimeric) (AB319022)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling GFAP with ab319022 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in rat primary glia cell (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab201732 Anti-GFAP rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/None dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Mouse IgG2a (Chimeric) (AB319022)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling GFAP with ab319022 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in mouse primary glia cell (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab201732 Anti-GFAP rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/None dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Mouse IgG2a (Chimeric) (AB319022)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh frozen) tissue labeling GFAP with ab319022 at 1/500 (1.0 ug/ml) dilution (Green).

Low expression : confocal image showing no staining on rat liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab319022 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Mouse IgG2a (Chimeric) (AB319022)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh frozen) tissue labeling GFAP with ab319022 at 1/500 (1.0 ug/ml) dilution (Green).

Low expression : confocal image showing no staining on mouse liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab319022 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).