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AB225314

Alexa Fluor® 488 Anti-GFP antibody [EPR14104] - GFP booster

2

(2 Reviews)

|

(3 Publications)

Anti-GFP antibody [EPR14104] - GFP booster - Alexa Fluor® 488 conjugated (ab225314) is a rabbit recombinant monoclonal antibody detecting GFP in ICC/IF.

- Biophysical QC for unrivalled batch-batch consistency

See more GFP antibodies and assay kits

View Alternative Names

Green fluorescent protein, GFP

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GFP antibody [EPR14104] - GFP booster (AB225314)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GFP antibody [EPR14104] - GFP booster (AB225314)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilised GFP-transfected 293 cells labelling GFP with ab225314 at 1/250 dilution. ab195889 was used to counterstain alpha tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

  • 660 APC

    APC Anti-GFP antibody [EPR14104]

  • 578 PE

    PE Anti-GFP antibody [EPR14104]

  • Unconjugated

    Anti-GFP antibody [EPR14104]

  • Carrier free

    Anti-GFP antibody [EPR14104] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14104

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }

Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-GFP antibody [EPR14104] - GFP booster (ab225314) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF). Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Related products
Antibody clone EPR14104 is also available pre-conjugated to a variety of labels for your convenience – Anti-GFP Alexa Fluor® 488 [EPR14104] - booster (ab225314).

Other related products
We have a range of other formats of antibody clone [EPR14104] also available for your convenience: ab183734, Carrier free - ab220802, Alexa Fluor® 488 - ab225314, APC - ab283597, ab300643, Carrier free - ab300644, PE - ab303588

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:3215 PubMed39863746

2025

The role of Box A of HMGB1 in producing γH2AX associated DNA breaks in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sirapat Settayanon,Pithi Chanvorachote,Apiwat Mutirangura

Nature communications 15:10011 PubMed39562549

2024

Nuclear receptor-SINE B1 network modulates expanded pluripotency in blastoids and blastocysts.

Applications

Unspecified application

Species

Unspecified reactive species

Ka Wai Wong,Yingying Zeng,Edison Tay,Jia Hao Jackie Teo,Nadia Omega Cipta,Kiyofumi Hamashima,Yao Yi,Haijun Liu,Tushar Warrier,Minh T N Le,Soon Chye Ng,Qi-Jing Li,Hu Li,Yuin-Han Loh

American journal of human genetics 111:1656-1672 PubMed39043182

2024

Functional characterization of 2,832 JAG1 variants supports reclassification for Alagille syndrome and improves guidance for clinical variant interpretation.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa A Gilbert,Ernest Keefer-Jacques,Tanaya Jadhav,Daniel Antfolk,Qianqian Ming,Nicolette Valente,Grace Tzun-Wen Shaw,Christopher J Sottolano,Grace Matwijec,Vincent C Luca,Kathleen M Loomes,Ramakrishnan Rajagopalan,Tristan J Hayeck,Nancy B Spinner
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com