AB195359

Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915]

Name: Alexa Fluor® 488 conjugated Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195359) | Abcam
Brand: Abcam Limited
SKU: AB195359
Price: 185 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(15 Publications)

Anti-Glucose Transporter GLUT1 antibody [EPR3915] - Alexa Fluor^® 488 conjugated (ab195359) is a rabbit recombinant monoclonal antibody detecting Glucose Transporter GLUT1 in Flow Cytometry (Intra), ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

GLUT1, SLC2A1, HepG2 glucose transporter, GLUT-1

2 Images

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB195359)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB195359)

Overlay histogram showing HepG2 cells stained with ab195359 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195359, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor^® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This antibody gave a positive signal in HepG2 fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB195359)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB195359)

ab195359 staining Glucose Transporter GLUT1 in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195359 at a working dilution of 1 in 50 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor^® 594, shown in red) at 2μg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Carrier free

Anti-Glucose Transporter GLUT1 antibody [EPR3915] - Low endotoxin, Azide free
Carrier free

Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free
Unconjugated

Anti-Glucose Transporter GLUT1 antibody [EPR3915]
660 APC

APC Anti-Glucose Transporter GLUT1 antibody [EPR3915]
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915]
Biotin

Biotin Anti-Glucose Transporter GLUT1 antibody [EPR3915]
519 FITC

FITC Anti-Glucose Transporter GLUT1 antibody [EPR3915]
HRP

HRP Anti-Glucose Transporter GLUT1 antibody [EPR3915]
578 PE

PE Anti-Glucose Transporter GLUT1 antibody [EPR3915]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3915

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/alexa-fluor-488-rabbit-igg-monoclonal-epr25a-isotype-control-ab199091'>ab199091</a> - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195359) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EPR3915 is also available pre-conjugated to a variety of labels for your convenience – Anti-Glucose Transporter GLUT1 Alexa Fluor® 488 [EPR3915] (ab195359).

Other related products
We have a range of other formats of antibody clone [EPR3915] also available for your convenience: ab115730, Alexa Fluor® 647 - ab195020, HRP - ab195021, Alexa Fluor® 488 - ab195359, Carrier free - ab196357, Alexa Fluor® 594 - ab206360, PE - ab209449, Alexa Fluor® 405 - ab210438, Carrier free - ab252403, APC - ab316298, FITC - ab322306, Biotin - ab323677

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Glucose Transporter GLUT1 also known as SLC2A1 is an important protein responsible for the transport of glucose across cell membranes. The GLUT1 transporter has a molecular weight of approximately 55 kDa. This protein is highly expressed in erythrocytes endothelial cells lining blood vessels and in the blood-brain barrier. Its primary role is to facilitate the basal glucose uptake necessary for cellular metabolism particularly in tissues where glucose is a critical energy source.

Biological function summary

This glucose transporter plays a significant role in maintaining glucose homeostasis in the human body. GLUT1 functions independently and not as part of a complex. It ensures that glucose is available to cells with high metabolic demands including the brain and red blood cells where it remains important for survival and function. Its expression level can be influenced by various factors including hypoxia and insulin.

Pathways

GLUT1 is involved in the glycolysis and hypoxia-related pathways. It supports the glycolytic pathway by ensuring a sufficient supply of glucose to the cells which is then metabolized to produce ATP. Additionally during hypoxic conditions GLUT1 expression can increase aligning with proteins like HIF-1α which helps cells adapt by modifying their metabolism. This coordinated regulation permits cells to adjust their energy systems according to the oxygen availability.

GLUT1 is implicated in glucose transporter type 1 deficiency syndrome (GLUT1 DS) and various forms of cancer. GLUT1 DS results from inadequate glucose transport into the brain presenting neurological symptoms due to energy deficiency. In cancer overexpression of GLUT1 links to increased glucose uptake and tumor growth a condition known to involve proteins like hexokinase. These associations underline GLUT1's contribution to both genetic defects and metabolic shifts in cancerous tissues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Facilitative glucose transporter, which is responsible for constitutive or basal glucose uptake (PubMed : 10227690, PubMed : 10954735, PubMed : 18245775, PubMed : 19449892, PubMed : 25982116, PubMed : 27078104, PubMed : 32860739). Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses (PubMed : 18245775, PubMed : 19449892). Most important energy carrier of the brain : present at the blood-brain barrier and assures the energy-independent, facilitative transport of glucose into the brain (PubMed : 10227690). In association with BSG and NXNL1, promotes retinal cone survival by increasing glucose uptake into photoreceptors (By similarity). Required for mesendoderm differentiation (By similarity).

See full target information SLC2A1

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1188 PubMed40781500

2025

Resynchronization of microglial activity in the brain is associated with restoration of motor function in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Peizhen Ye,Lei Bi,Yifan Qiu,Min Yang,Yongshan Liu,Yuyi Hou,Pengcheng Zheng,Xiaojuan Cao,Jing Su,Hongjun Jin

iScience 28:111994 PubMed40071147

2025

IQGAP2 regulates blood-brain barrier immune dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Ketaki A Katdare,Andrew Kjar,Natasha M O'Brown,Emma H Neal,Alexander G Sorets,Alena Shostak,Wilber Romero-Fernandez,Alexander J Kwiatkowski,Kate Mlouk,Hyosung Kim,Rebecca P Cowell,Katrina R Schwensen,Cristian O Carvajal Tapia,Guste Venslovaite,Kensley B Horner,John T Wilson,Matthew S Schrag,Sean G Megason,Ethan S Lippmann

Cell reports. Medicine 5:101869 PubMed39657666

2024

Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state.

Applications

Unspecified application

Species

Unspecified reactive species

Xingying Zhang,Chenze Zhang,Shan Lu,Jingxi Dong,Na Tang,Yao Wang,Weidong Han,Xi Pan,Xiang Zhang,Duan Liu,Ng Shyh-Chang,Yu Wang,Guihai Feng,Haoyi Wang

iScience 27:110662 PubMed39252969

2024

Single-cell transcriptomics reveals subset-specific metabolic profiles underpinning the bronchial epithelial response to flagellin.

Applications

Unspecified application

Species

Unspecified reactive species

Ivan Ramirez-Moral,Alex R Schuurman,Christine C A van Linge,Joe M Butler,Xiao Yu,Karen de Haan,Sarah van Leeuwen,Alex F de Vos,Menno D de Jong,Felipe A Vieira Braga,Tom van der Poll

Journal of translational medicine 22:5 PubMed38169393

2024

PIGT promotes cell growth, glycolysis, and metastasis in bladder cancer by modulating GLUT1 glycosylation and membrane trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyue Tan,Qi Pan,Chao Yu,Xinyu Zhai,Jianyi Gu,Le Tao,Dongliang Xu

BioMed research international 2022:4271358 PubMed35924266

2022

Alpha-Amylase Inhibits Cell Proliferation and Glucose Uptake in Human Neuroblastoma Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Kateryna Pierzynowska,Sofia Thomasson,Stina Oredsson

Nature communications 13:1789 PubMed35379825

2022

Glutathione-dependent redox balance characterizes the distinct metabolic properties of follicular and marginal zone B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Davide G Franchina,Henry Kurniawan,Melanie Grusdat,Carole Binsfeld,Luana Guerra,Lynn Bonetti,Leticia Soriano-Baguet,Anouk Ewen,Takumi Kobayashi,Sophie Farinelle,Anna Rita Minafra,Niels Vandamme,Anaïs Carpentier,Felix K Borgmann,Christian Jäger,Ying Chen,Markus Kleinewietfeld,Vasilis Vasiliou,Michel Mittelbronn,Karsten Hiller,Philipp A Lang,Dirk Brenner

Cell host & microbe 30:530-544.e6 PubMed35316647

2022

Fungal sensing enhances neutrophil metabolic fitness by regulating antifungal Glut1 activity.

Applications

Unspecified application

Species

Unspecified reactive species

De-Dong Li,Chetan V Jawale,Chunsheng Zhou,Li Lin,Giraldina J Trevejo-Nunez,Syed A Rahman,Steven J Mullet,Jishnu Das,Stacy G Wendell,Greg M Delgoffe,Michail S Lionakis,Sarah L Gaffen,Partha S Biswas

Cell reports 34:108831 PubMed33691103

2021

Blockade of 6-phosphogluconate dehydrogenase generates CD8 effector T cells with enhanced anti-tumor function.

Applications

Unspecified application

Species

Unspecified reactive species

Saeed Daneshmandi,Teresa Cassel,Penghui Lin,Richard M Higashi,Gerburg M Wulf,Vassiliki A Boussiotis,Teresa W-M Fan,Pankaj Seth

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 70:473-481 PubMed33751130

2021

Propofol regulates activated macrophages metabolism through inhibition of ROS-mediated GLUT1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zeng,Zeting Xing,Meiyun Tan,Yanwen Wu,Chunyuan Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915]

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB195359)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB195359)

Related conjugates and formulations (11)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise