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AB202292

Alexa Fluor® 488 Anti-GNAI1 antibody [EPR9441(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal GNAI1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Guanine nucleotide-binding protein G(i) subunit alpha-1, Adenylate cyclase-inhibiting G alpha protein, GNAI1

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GNAI1 antibody [EPR9441(B)] (AB202292)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GNAI1 antibody [EPR9441(B)] (AB202292)

ab202292 staining GNAI1 in SK-N-SH cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202292 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in SK-N-SH cells fixed with 100% methanol (5min).

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-GNAI1 antibody [EPR9441(B)] (AB202292)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-GNAI1 antibody [EPR9441(B)] (AB202292)

Overlay histogram showing SH-SY5Y cells stained with ab202292 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab202292, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9441(B)

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p>This product gave a positive signal in SK-N-SH cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein GNAI1 also known as G protein subunit alpha i1 is part of the G-protein family that plays an important role in signal transduction. GNAI1 has a molecular mass of approximately 40 kDa. It is mainly expressed in neuronal tissues where it interacts with receptors to regulate intracellular signaling pathways. This protein operates by inhibiting adenylate cyclase activity which reduces the production of cyclic AMP (cAMP) in response to extracellular signals.
Biological function summary

GNAI1 acts by interfering with signal transmission across membranes. It is part of the heterotrimeric G-protein complex which is essential for many signaling processes. This complex responds to activation by G-protein-coupled receptors (GPCRs) and modulates various downstream effectors essentially affecting numerous physiological processes like neurotransmission and hormone signaling.

Pathways

GNAI1 plays a significant role in the cyclic AMP (cAMP) signaling pathway and the neuroactive ligand-receptor interaction pathway. In the cAMP pathway GNAI1 associates with receptors like dopamine and serotonin receptors helping to regulate neuronal response and neurotransmitter release. This activity involves interactions with related proteins such as GNB1 and GNG2 which form the beta and gamma subunits of the heterotrimeric G-protein complex.

GNAI1 relates to neuropathic conditions such as epilepsy and depression. Alterations in GNAI1 function can disrupt normal signaling contributing to these disorders. The protein interacts with other proteins in the signaling cascade such as RGS proteins which can affect its function in disease states. Researchers continue to explore how modifications or mutations in GNAI1 might offer potential therapeutic targets for these and similar disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed : 18434541, PubMed : 33762731, PubMed : 34239069, PubMed : 35610220, PubMed : 37935376, PubMed : 37935377, PubMed : 37963465, PubMed : 38552625, PubMed : 8774883). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 18434541, PubMed : 8774883). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 18434541, PubMed : 8774883). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 18434541, PubMed : 8774883). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 18434541, PubMed : 8774883). Signaling is mediated via effector proteins, such as adenylate cyclase : inhibits adenylate cyclase activity of ADCY1, ADCY5 and ADCY6, leading to decreased intracellular cAMP levels (PubMed : 8119955). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis (PubMed : 17635935). Required for cortical dynein-dynactin complex recruitment during metaphase (PubMed : 22327364).
See full target information GNAI1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:224 PubMed36990998

2023

Targeting Aurora-A inhibits tumor progression and sensitizes thyroid carcinoma to Sorafenib by decreasing PFKFB3-mediated glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Jingtai,Hu Linfei,Qian Yuyang,Kang Ning,Yun Xinwei,Wang Xin,Ruan Xianhui,Huang Dongmei,Yang Weiwei,Meng Xiangrui,Zhu Tianze,Wang Wei,Zheng Xiangqian
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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