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AB325374

Alexa Fluor® 488 Anti-GOLM1/GOLPH2 antibody [EPR29022-61]

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Rabbit Recombinant Monoclonal GOLPH2 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, ICC/IF and reacts with Human samples.

View Alternative Names

C9orf155, GOLPH2, PSEC0242, UNQ686/PRO1326, GOLM1, Golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-GOLM1/GOLPH2 antibody [EPR29022-61] (AB325374)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-GOLM1/GOLPH2 antibody [EPR29022-61] (AB325374)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling GOLM1/GOLPH2 with ab325374 at 1/50 (10.0 ug/ml) dilution (green).

Positive staining on human colon cancer.

The primary antibody was incubated overnight at 4°C (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Dilution buffer only control.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-GOLM1/GOLPH2 antibody [EPR29022-61] (AB325374)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-GOLM1/GOLPH2 antibody [EPR29022-61] (AB325374)

Immunohistochemical analysis of paraffin-embedded Human appendix tissue labeling GOLM1/GOLPH2 with ab325374 at 1/50 (10.0 ug/ml) dilution (green).

Positive staining on human appendix.

The primary antibody was incubated overnight at 4°C (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Dilution buffer only control.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GOLM1/GOLPH2 antibody [EPR29022-61] (AB325374)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GOLM1/GOLPH2 antibody [EPR29022-61] (AB325374)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized GOLM1 KO HEK293T (GOLM1 knockout human embryonic kidney epithelial cell) and Wildtype HEK-293T (human embryonic kidney epithelial cell) cells labelling GOLM1/GOLPH2 with ab325374 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing golgi staining in parental HEK293T cell line (shown in green) and no staining in GOLM1 knockout HEK-293T cell line. The counterstain was golgi marker which was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker was used to counterstain golgi at 1/200 (5 ug/ml) dilution followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-GOLM1/GOLPH2 antibody [EPR29022-61] (AB325374)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-GOLM1/GOLPH2 antibody [EPR29022-61] (AB325374)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling GOLM1/GOLPH2 with ab325374 at 1/50 (10.0 ug/ml) dilution (green).

Negative control : no staining on human skeletal muscle.

The primary antibody was incubated overnight at 4°C (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Unconjugated

    Anti-GOLM1/GOLPH2 antibody [EPR29022-61]

  • Carrier free

    Anti-GOLM1/GOLPH2 antibody [EPR29022-61] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29022-61

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GOLPH2 also known as GOLM1 or Golgi membrane protein 1 has a significant mechanical role in the cell. It is a 73-kilodalton protein found mainly in the Golgi apparatus. This protein gets expressed in various tissues with notable levels in the liver and other organs involved in secretion. It acts as a transmembrane protein in the Golgi where it helps in processing and sorting secretory proteins influencing how proteins move through secretory pathways.
Biological function summary

GOLPH2 plays an essential role in maintaining Golgi function and efficient protein trafficking. It does not belong to a large protein complex but its proper functioning is critical for handling secretory pathways. The protein keeps secretory flow organized which affects cellular responses and interactions with the environment. The GOLPH2 protein also has connections to immunity responding to stimuli like infections or inflammation.

Pathways

GOLPH2 actively participates in secretory pathways and protein transport systems. Its role includes coordination with other Golgi proteins to facilitate protein modification and sorting. GOLPH2 interacts with other proteins like GOLM1 and GOLPH3 impacting the Golgi's structural organization and influencing pathways like vesicular transport. These interactions are important for adapting the intracellular environment to external demands.

GOLPH2 levels change in diseases such as liver cancer and hepatitis. These changes can serve as biomarkers for diagnosis and disease progression monitoring. GOLPH2 also connects with proteins involved in these diseases including AFP a known tumor marker. Understanding GOLPH2's role in such conditions aids research in developing diagnostic tools and therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Unknown. Cellular response protein to viral infection.
See full target information GOLM1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com