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Rabbit Recombinant Monoclonal Granzyme B antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra) and reacts with Mouse samples.

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Images

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Granzyme B antibody [EPR22645-244] (AB307609), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)ICC/IF
Mouse
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed:35705808). It cleaves after Asp (PubMed:35705808). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (By similarity). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution (By similarity). Cleaves caspase-3 and -9 (CASP3 and CASP9, respectively) to give rise to active enzymes mediating apoptosis (PubMed:35705808). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (PubMed:35705808).

Alternative names

Ctla-1, Ctla1, Gzmb, CTLA-1, Cytotoxic cell protease 1, Fragmentin-2, CCP1

Recommended products

Rabbit Recombinant Monoclonal Granzyme B antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra) and reacts with Mouse samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22645-244
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Granzyme B antibody [EPR22645-244] (ab307609), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Granzyme B antibody [EPR22645-244] (ab307609)

    Flow cytometric analysis of Mouse splenocyte treated with 2.5 μg/ml Concanavalin A for 72h cells labelling Granzyme B with ab307609 at 1/500 dilution (0.1µg)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor ® 488) (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody (Blue).  Live cells were stained with anti-CD8a conjugated to Alexa Fluor ® 647. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or ab307609.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com