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Mouse Monoclonal GRIM19 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Mouse samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GRIM19 antibody [6E1BH7] (AB197965), expandable thumbnail

Key facts

Isotype
IgG2b
Host species
Mouse
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Mouse
Tested

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

This product gave a positive signal in MEF1 cells fixed with 100% methanol (5 min).

Associated Products

Select an associated product type

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Target data

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis (PubMed:27626371). Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (PubMed:27626371). Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes (PubMed:15753091).

Alternative names

GRIM19, CDA016, CGI-39, NDUFA13, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13, Cell death regulatory protein GRIM-19, Complex I-B16.6, Gene associated with retinoic and interferon-induced mortality 19 protein, NADH-ubiquinone oxidoreductase B16.6 subunit, CI-B16.6, GRIM-19, Gene associated with retinoic and IFN-induced mortality 19 protein

Recommended products

Mouse Monoclonal GRIM19 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Mouse samples.

Key facts

Isotype
IgG2b
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
6E1BH7
Purification technique
Affinity purification
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GRIM19 also known as NDUFA13 is a small protein with a molecular mass of approximately 16 kDa. It expresses mainly in mitochondria where it forms an integral component of the mitochondrial respiratory chain complex I. GRIM19 plays an important role in the functioning of complex I by facilitating the assembly and stabilization of the complex enabling efficient electron transport and ATP synthesis.

Biological function summary

GRIM19 interacts with several complexes and proteins within the mitochondrial inner membrane. As a part of complex I also known as NADH:ubiquinone oxidoreductase it contributes to the initial step of mitochondrial oxidative phosphorylation. GRIM19 also participates in the regulation of apoptosis and its expression is influenced during cellular stress situations highlighting its significance in cellular survival and energy production.

Pathways

GRIM19 integrates closely with mitochondrial apoptosis and energy metabolism pathways. Its interactions within oxidative phosphorylation place it in concert with both the electron transport chain and the apoptosis regulatory network. GRIM19 shares associations with related proteins such as cytochrome c oxidase and other complex I subunits highlighting its essential contribution to these important biological processes.

Associated diseases and disorders

GRIM19 demonstrates links to various pathologies including cancer and mitochondrial diseases. Alterations in GRIM19 expression or function have been implicated in the progression of certain cancers such as colorectal and thyroid cancer where GRIM19's usual regulatory role in apoptosis can be disrupted. Additionally its involvement in mitochondrial disease connects to proteins like COX-A1 emphasizing the importance of maintaining functional complexes for cellular health.

Product promise

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1 product image

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GRIM19 antibody [6E1BH7] (ab197965), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GRIM19 antibody [6E1BH7] (ab197965)

    ab197965 staining GRIM19 in MEF1 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197965 at a 1/50 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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