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AB204258

Alexa Fluor® 488 Anti-Histone H2B antibody [mAbcam 52484]

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(2 Publications)

Mouse Monoclonal H2B antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

H2BFF, HIST1H2BB, H2BC3, Histone H2B type 1-B, H2B-clustered histone 3, Histone H2B.1, Histone H2B.f, H2B/f

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H2B antibody [mAbcam 52484] (AB204258)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H2B antibody [mAbcam 52484] (AB204258)

ab204258 staining Histone H2B in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204258 at 1/2000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Unconjugated

    Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade

  • Carrier free

    Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade - BSA and Azide free

  • HRP

    HRP Anti-Histone H2B antibody [mAbcam 52484]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Histone H2B antibody [mAbcam 52484]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam 52484

Isotype

IgG1

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed 100% methanol (5 min).</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purity
IgG fraction
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H2BC3

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 17:1687-1706 PubMed38504693

2024

Envoplakin Inhibits Macrophage Polarization by Altering the Inflammatory Tumor Microenvironment of Melanoma Through the RAS / ERK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weilin Cai,Minliang Chen

Stem cell research & therapy 10:349 PubMed31775893

2019

Flow-enhanced priming of hESCs through H2B acetylation and chromatin decondensation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawen Wang,Yi Wu,Xiao Zhang,Fan Zhang,Dongyuan Lü,Bing Shangguan,Yuxin Gao,Mian Long
View all publications

Product promise

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