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Rabbit Recombinant Monoclonal H3 acetyl K9 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Mouse, Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (acetyl K9) antibody [Y28] (AB200986), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (acetyl K9) antibody [Y28] (AB200986), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested
Mouse
Tested
Rat
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Species

Human

Dilution info

1/100

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

    9 products for Alternative Product

      Target data

      Function

      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      Alternative names

      Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3C7, H3C11, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, HIST1H3J, H3FJ, H3C12, HIST1H3I, H3FF, H3FD, H3FL, H3C2, HIST1H3A, H3FA, H3C1, HIST1H3E, HIST1H3B, H3FC, HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3C3, H3K9ac

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      Rabbit Recombinant Monoclonal H3 acetyl K9 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Mouse, Human samples.

      Alternative names

      Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3C7, H3C11, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, HIST1H3J, H3FJ, H3C12, HIST1H3I, H3FF, H3FD, H3FL, H3C2, HIST1H3A, H3FA, H3C1, HIST1H3E, HIST1H3B, H3FC, HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3C3, H3K9ac

      Key facts

      Isotype

      IgG

      Conjugation

      Alexa Fluor® 488

      Excitation/Emission

      Ex: 495nm, Em: 519nm

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      Y28

      Purification technique

      Affinity purification Protein A

      Concentration
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      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle, Store in the dark

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      2 product images

      • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (acetyl K9) antibody [Y28] (ab200986), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (acetyl K9) antibody [Y28] (ab200986)

        ab200986 staining Histone H3 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4C with ab200986 at 1/100 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594) at 1/200 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

        This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

      • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (acetyl K9) antibody [Y28] (ab200986), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (acetyl K9) antibody [Y28] (ab200986)

        ab200986 staining Histone H3 in NIH-3T3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200986 at 1/100 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594) at 1/200 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

      For licensing inquiries, please contact partnerships@abcam.com