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AB310967

Alexa Fluor® 488 Anti-HuR / ELAVL1 antibody [EPR17397]

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Rabbit Recombinant Monoclonal HuR / ELAVL1 antibody - conjugated to Alexa Fluor® 488.

View Alternative Names

HUR, ELAVL1, ELAV-like protein 1, Hu-antigen R, HuR

  • Unconjugated

    Anti-HuR / ELAVL1 antibody [EPR17397]

  • Carrier free

    Anti-HuR / ELAVL1 antibody [EPR17397] - BSA and Azide free

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-HuR / ELAVL1 antibody [EPR17397]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-HuR / ELAVL1 antibody [EPR17397]

  • 660 APC

    APC Anti-HuR / ELAVL1 antibody [EPR17397]

  • 578 PE

    PE Anti-HuR / ELAVL1 antibody [EPR17397]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-HuR / ELAVL1 antibody [EPR17397]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-HuR / ELAVL1 antibody [EPR17397]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-HuR / ELAVL1 antibody [EPR17397]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17397

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HuR also known as ELAVL1 is a protein that binds RNA in cells. It weighs about 36 kDa and is found in the nucleus and cytoplasm. Its name HuR comes from its similarity to human antigen R. Researchers often call it the HuR protein or ELAVL1 protein. This protein helps stabilize certain mRNA molecules by binding to AU-rich elements (AREs) in their 3' untranslated regions. Its binding affects mRNA decay rates and therefore influences protein synthesis.
Biological function summary

HuR influences cell growth differentiation and stress responses. It does not work alone; it often forms part of larger ribonucleoprotein complexes. By stabilizing mRNAs HuR ensures the continuous production of proteins necessary for cell survival and adaptation to stressors. This regulatory role highlights its involvement in many cellular processes making it a focus for study in relation to cellular homeostasis.

Pathways

HuR is part of the mRNA surveillance pathway and the response to cellular stress. HuR modulates gene expression by interacting with proteins involved in transcription and translation regulation. It frequently partners with proteins like TTP (tristetraprolin) that also bind to AREs affecting mRNA stability and translation processes. Such interactions extend HuR's influence across pathways related to gene expression regulation and response to stress.

Researchers associate HuR with cancer and neurodegenerative diseases. In cancer HuR stabilizes mRNAs of many oncogenes aiding tumor growth and progression. It may interact with proteins like p53 affecting tumor suppressor pathways. For neurodegenerative diseases HuR's role in mRNA stability can influence neural health connected to proteins like TDP-43 in conditions such as Alzheimer's and ALS. Understanding HuR's function offers insights into potential therapeutic targets for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed : 14517288, PubMed : 18285462, PubMed : 31358969). Involved in embryonic stem cell (ESC) differentiation : preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation (By similarity). Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed : 32245947). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed : 14731398, PubMed : 17632515, PubMed : 18285462, PubMed : 23519412, PubMed : 8626503). Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed : 8626503). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR (PubMed : 29180010).
See full target information ELAVL1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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