AB225260

Alexa Fluor® 488 Anti-Iba1 antibody [EPR16588] - Microglia marker

Name: Alexa Fluor® 488 conjugated Anti-Iba1 antibody [EPR16588] (ab225260) | Abcam
Brand: Abcam Limited
SKU: AB225260
Price: 585 USD
Availability: InStock
Rating: 4 (6 reviews)

RabMAb
Recombinant
What is this?

(6 Reviews)

(6 Publications)

Anti-Iba1 antibody [EPR16588] - Alexa Fluor® 488 conjugated (ab225260) is a rabbit recombinant monoclonal antibody detecting Iba1 in IHC-P. Suitable for Human.

- Clone EPR16588 is the most cited clone to Iba1
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

G1, IBA1, AIF1, Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1, iba1, iba-1, iba 1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Iba1 antibody [EPR16588] - Microglia marker (AB225260)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Iba1 antibody [EPR16588] - Microglia marker (AB225260)

IHC image of Iba1 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110^oC for 20 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab225260 at 1/100 dilution (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Iba1 antibody [EPR16588] - Microglia marker
660 APC

APC Anti-Iba1 antibody [EPR16588] - Microglia marker
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Iba1 antibody [EPR16588] - Microglia marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16588

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-Iba1 antibody [EPR16588] (ab225260) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Related products
Antibody clone EPR16588 is also available pre-conjugated to a variety of labels for your convenience – Anti-Iba1 Alexa Fluor® 488 [EPR16588] (ab225260).

Other related products
We have a range of other formats of antibody clone [EPR16588] also available for your convenience: ab178846, Carrier free - ab220815, Alexa Fluor® 488 - ab225260, Alexa Fluor® 647 - ab225261, ab300156, Carrier free - ab300157, ab318302, Carrier free - ab318305, Alexa Fluor® 750 - ab321218

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Iba1 also known as Allograft Inflammatory Factor 1 (AIF-1) is a 17 kDa protein notable for its involvement in immune responses. It is expressed mainly in microglia and macrophages key components of the central nervous system's immune system. Researchers often use Iba1 in Western blots and immunohistochemistry (IHC) to study microglial activation. The protein's expression is a strong marker of microglial activation making it a focal point in neuroinflammation studies. Anti-Iba1 antibodies have gained popularity for their effectiveness in identifying microglia in brain tissue sections through Iba1 staining techniques.

Biological function summary

Iba1 modulates actin bundling facilitating changes in microglial morphology and motility. It does not form part of a multiprotein complex but is significantly involved in cytoskeletal dynamics. This protein's expression increases during brain injury indicating its role in response to neural trauma. Iba1 has calcium-binding properties which suggest that it might interact with proteins involved in calcium signaling pathways.

Pathways

Calcium signaling and actin cytoskeletal rearrangement are significant biological processes that involve Iba1. Within these pathways Iba1 interacts closely with other actin-binding proteins that affect microglial movement and morphology. Such interactions illustrate how Iba1 helps microglia navigate and respond to changes within the brain environment.

Iba1 is often associated with neurodegenerative diseases like Alzheimer's disease and amyotrophic lateral sclerosis (ALS). In these conditions elevated Iba1 levels mark increased microglial activity which correlates with disease progression. Connections with proteins such as amyloid-beta in Alzheimer's disease suggest its role in mediating inflammatory responses and linking inflammation to disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

See full target information AIF1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Biology of sex differences 16:29 PubMed40302001

2025

ERβ mediates sex-specific protection in the App-NL-G-F mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Aphrodite Demetriou,Birgitta Lindqvist,Heba G Ali,Mohamed M Shamekh,Mukesh Varshney,Jose Inzunza,Silvia Maioli,Per Nilsson,Ivan Nalvarte

Methods in molecular biology (Clifton, N.J.) 2857:61-77 PubMed39348055

2024

Investigation of Signaling in Primary Populations of Human Senescent T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alessio Lanna,Federica Rinaldi

Experimental neurobiology 32:343-353 PubMed37927132

2023

BDNF/TrkB Signaling Inhibition Suppresses Astrogliosis and Alleviates Mechanical Allodynia in a Partial Crush Injury Model.

Applications

Unspecified application

Species

Unspecified reactive species

Tien Thuy Phan,Nishani Jayanika Jayathilake,Kyu Pil Lee,Joo Min Park

The European journal of neuroscience 58:4107-4122 PubMed37846812

2023

Long non-coding RNA MIR17HG impedes FOSL2-mediated transcription activation of HIC1 to maintain a pro-inflammatory phenotype of microglia during intracerebral haemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Yunzheng Ai,Ying Kong,Zheng Zou,Ligang Chen,Guobiao Liang

Journal of photochemistry and photobiology. B, Biology 216:112152 PubMed33610085

2021

Gut flora-targeted photobiomodulation therapy improves senile dementia in an Aß-induced Alzheimer's disease animal model.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Chen,Jinpeng Wu,Xiaoxi Dong,Huijuan Yin,Xiafei Shi,Siying Su,Bochen Che,Yingxin Li,Jichun Yang

Blood advances 4:4366-4381 PubMed32926126

2020

Interferon-β alleviates delayed tPA-induced adverse effects via modulation of MMP3/9 production in ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Ping-Chang Kuo,Wen-Tsan Weng,Barbara A Scofield,Destin Furnas,Hallel C Paraiso,Alexander J Intriago,Kristopher D Bosi,I-Chen Yu,Jui-Hung Yen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-Iba1 antibody [EPR16588] - Microglia marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Iba1 antibody [EPR16588] - Microglia marker (AB225260)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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