AB195031

Alexa Fluor® 488 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker

RabMAb
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal Iba1 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.

View Alternative Names

G1, IBA1, AIF1, Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1, iba1, iba-1, iba 1

1 Images

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker (AB195031)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker (AB195031)

Overlay histogram showing K562 cells stained with ab195031 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195031, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluorr^® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

578 PE

PE Anti-Iba1 antibody [EPR6136(2)] - Microglia marker
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker
Unconjugated

Anti-Iba1 antibody [EPR6136(2)] - Microglia marker
Carrier free

Anti-Iba1 antibody [EPR6136(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6136(2)

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Iba1 also known as Allograft Inflammatory Factor 1 (AIF-1) is a 17 kDa protein notable for its involvement in immune responses. It is expressed mainly in microglia and macrophages key components of the central nervous system's immune system. Researchers often use Iba1 in Western blots and immunohistochemistry (IHC) to study microglial activation. The protein's expression is a strong marker of microglial activation making it a focal point in neuroinflammation studies. Anti-Iba1 antibodies have gained popularity for their effectiveness in identifying microglia in brain tissue sections through Iba1 staining techniques.

Biological function summary

Iba1 modulates actin bundling facilitating changes in microglial morphology and motility. It does not form part of a multiprotein complex but is significantly involved in cytoskeletal dynamics. This protein's expression increases during brain injury indicating its role in response to neural trauma. Iba1 has calcium-binding properties which suggest that it might interact with proteins involved in calcium signaling pathways.

Pathways

Calcium signaling and actin cytoskeletal rearrangement are significant biological processes that involve Iba1. Within these pathways Iba1 interacts closely with other actin-binding proteins that affect microglial movement and morphology. Such interactions illustrate how Iba1 helps microglia navigate and respond to changes within the brain environment.

Iba1 is often associated with neurodegenerative diseases like Alzheimer's disease and amyotrophic lateral sclerosis (ALS). In these conditions elevated Iba1 levels mark increased microglial activity which correlates with disease progression. Connections with proteins such as amyloid-beta in Alzheimer's disease suggest its role in mediating inflammatory responses and linking inflammation to disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

See full target information AIF1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 14:e70707 PubMed40028673

2025

Secretomes From Non-Small Cell Lung Cancer Cells Induce Endothelial Plasticity Through a Partial Endothelial-to-Mesenchymal Transition.

Applications

Unspecified application

Species

Unspecified reactive species

Clara Bourreau,Emilie Navarro,Marine Cotinat,Morgane Krejbich,François Guillonneau,Catherine Guette,Alice Boissard,Cécile Henry,Isabelle Corre,Lucas Treps,Nicolas Clere

Progress in biophysics and molecular biology 168:3-9 PubMed34536443

2021

Exploring the human cerebral cortex using confocal microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Pesce,Annunziatina Laurino,Marina Scardigli,Jiarui Yang,David A Boas,Patrick R Hof,Christophe Destrieux,Irene Costantini,Francesco Saverio Pavone

Biomedical optics express 12:3684-3699 PubMed34221688

2021

Large-scale, cell-resolution volumetric mapping allows layer-specific investigation of human brain cytoarchitecture.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Costantini,Giacomo Mazzamuto,Matteo Roffilli,Annunziatina Laurino,Filippo Maria Castelli,Mattia Neri,Giovanni Lughi,Andrea Simonetto,Erica Lazzeri,Luca Pesce,Christophe Destrieux,Ludovico Silvestri,Valerio Conti,Renzo Guerrini,Francesco Saverio Pavone

Journal of tissue science & engineering 12: PubMed39055131

2021

Whole-Cell Dissociated Suspension Analysis in Human Brain Neurodegenerative Disease: A Pilot Study.

Applications

Unspecified application

Species

Unspecified reactive species

Geidy E Serrano,Jessica E Walker,Anthony J Intorcia,Michael J Glass,Richard A Arce,Ignazio S Piras,Joshua S Talboom,Courtney M Nelson,Brett D Cutler,Lucia I Sue,Lih-Fen Lue,Matthew Huentelman,Thomas G Beach

Cell 183:1354-1366.e13 PubMed33065030

2020

Vascular Disease and Thrombosis in SARS-CoV-2-Infected Rhesus Macaques.

Applications

Unspecified application

Species

Unspecified reactive species

Malika Aid,Kathleen Busman-Sahay,Samuel J Vidal,Zoltan Maliga,Stephen Bondoc,Carly Starke,Margaret Terry,Connor A Jacobson,Linda Wrijil,Sarah Ducat,Olga R Brook,Andrew D Miller,Maciel Porto,Kathryn L Pellegrini,Maria Pino,Timothy N Hoang,Abishek Chandrashekar,Shivani Patel,Kathryn Stephenson,Steven E Bosinger,Hanne Andersen,Mark G Lewis,Jonathan L Hecht,Peter K Sorger,Amanda J Martinot,Jacob D Estes,Dan H Barouch

Scientific data 6:323 PubMed31848351

2019

Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer.

Applications

mIHC

Species

Human

Rumana Rashid,Giorgio Gaglia,Yu-An Chen,Jia-Ren Lin,Ziming Du,Zoltan Maliga,Denis Schapiro,Clarence Yapp,Jeremy Muhlich,Artem Sokolov,Peter Sorger,Sandro Santagata

Nature protocols 14:2900-2930 PubMed31534232

2019

Qualifying antibodies for image-based immune profiling and multiplexed tissue imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Ziming Du,Jia-Ren Lin,Rumana Rashid,Zoltan Maliga,Shu Wang,Jon C Aster,Benjamin Izar,Peter K Sorger,Sandro Santagata

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker (AB195031)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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