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Mouse Monoclonal ICAM3 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing ICAM3 protein.

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Images

Flow Cytometry - Alexa Fluor® 488 Anti-ICAM3 antibody [MEM-171] (AB239270), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing ICAM3 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P32942

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species

Human

Dilution info

4 µL for 106 Cells

Notes

(or 100 μl of whole blood)

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed:1448173). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590).

Alternative names

Recommended products

Mouse Monoclonal ICAM3 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing ICAM3 protein.

Key facts

Isotype

IgG1

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing ICAM3 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P32942
Clone number

MEM-171

Purification technique

Size-exclusion chromatography

Concentration
Loading...
Purification notes

The purified antibody is conjugated with Alexa Fluor® 488 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ICAM-3 also known as CD50 or Intercellular Adhesion Molecule 3 is a cell surface glycoprotein. It weighs approximately 120 kilodaltons. ICAM-3 is expressed majorly on leukocytes including lymphocytes monocytes and granulocytes. It is a member of the immunoglobulin superfamily that participates in cell-cell interaction particularly in immune response. By engaging with integrin receptors mainly LFA-1 ICAM-3 plays a role in leukocyte adhesion and signaling.

Biological function summary

ICAM-3 contributes to the immune system’s functionality by mediating intercellular adhesion and cellular signaling. It acts as a ligand for the integrin LFA-1 found on leukocytes which facilitates the initial immune response. ICAM-3 is not a part of a larger complex but works closely with LFA-1 in homing and recruitment of immune cells to inflammation sites. Its interaction with LFA-1 also supports the formation of the immunological synapse an important step in T-cell activation and immune signaling.

Pathways

ICAM-3 contributes to both the immune response and leukocyte extravasation pathways. The interaction with LFA-1 places it within the context of immune cell signaling pathways impacting integrin-mediated signaling. Another pathway involving ICAM-3 is the cell adhesion pathway which is essential for maintaining proper immune surveillance and response. It collaborates with proteins like ICAM-1 in these pathways to ensure effective immune activity.

Associated diseases and disorders

ICAM-3 is associated with conditions such as inflammatory diseases and certain autoimmune disorders. Its expression levels and binding interactions can affect the severity and progression of such diseases. In autoimmune diseases ICAM-3 interaction with LFA-1 might contribute to the aberrant immune activation observed. Additionally studies have highlighted its role in infections where viruses exploit ICAM proteins to enter or spread showing its importance in pathogenesis-related conditions.

Product promise

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1 product image

  • Flow Cytometry - Alexa Fluor® 488 Anti-ICAM3 antibody [MEM-171] (ab239270), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Anti-ICAM3 antibody [MEM-171] (ab239270)

    Flow cytometric analysis of human peripheral blood cells labeling ICAM3 using ab239270. Surface staining. Gated on leukocytes and platelets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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