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AB313754

Alexa Fluor® 488 Anti-IgG2 antibody [EPR4418]

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Rabbit Recombinant Monoclonal IGHG2 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Immunoglobulin heavy constant gamma 2, Ig gamma-2 chain C region, Ig gamma-2 chain C region DOT, Ig gamma-2 chain C region TIL, Ig gamma-2 chain C region ZIE, IGHG2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-IgG2 antibody [EPR4418] (AB313754)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-IgG2 antibody [EPR4418] (AB313754)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling IgG2 with ab313754 at 1/100 (5.0 ug/ml) . Positive staining on the lamina propria of human colon. The section was incubated with ab313754 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-IgG2 antibody [EPR4418] (AB313754)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-IgG2 antibody [EPR4418] (AB313754)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling IgG2 with ab313754 at 1/100 (5.0 ug/ml). Cytoplasmic staining on human tonsil. The section was incubated with ab313754 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IgG2 antibody [EPR4418] (AB313754)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IgG2 antibody [EPR4418] (AB313754)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labelling IgG2 with ab313754 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Unconjugated

    Anti-IgG2 antibody [EPR4418]

  • Carrier free

    Anti-IgG2 antibody [EPR4418] - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-IgG2 antibody [EPR4418]

  • Biotin

    Biotin Anti-IgG2 antibody [EPR4418]

  • HRP

    HRP Anti-IgG2 antibody [EPR4418]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4418

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Human

Applications

IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB109489

Anti-Human IgG antibody [EPR4421]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (AB109489)

  • 4
  • 3
Secondary Antibodies

AB125912

Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L

RabMAb|Recombinant

ELISA - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (AB125912)

  • 0
  • 0
Secondary Antibodies

AB99817

Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP)

ELISA - Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP) (AB99817)

  • 0
  • 0
Secondary Antibodies

AB99779

Mouse monoclonal [31-7-4] Anti-Human IgG2 Fc (HRP)

ELISA - Mouse monoclonal [31-7-4] Anti-Human IgG2 Fc (HRP) (AB99779)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IgG2 also known as Immunoglobulin G2 is a subclass of the IgG antibodies within the immune system. It is a glycoprotein with a molecular mass typically around 150 kDa. As it is part of the IgG class IgG2 contributes to the body's defense mechanisms by binding to specific antigens. The expression of IgG2 occurs in plasma cells derived from B cells following antigen exposure. It circulates in the blood and extracellular fluid playing an important role in identifying and neutralizing pathogens such as bacteria and viruses.
Biological function summary

IgG2 plays an essential role in the immune response by activating the complement system therefore facilitating opsonization and eventual phagocytosis of pathogens. IgG2 forms part of the immunoglobulin family complex which mediates immune responses. It exhibits lower affinity for Fc receptors on phagocytic cells compared to other IgG subclasses. This characteristic affects its ability to mediate antibody-dependent cellular cytotoxicity. Despite this IgG2 remains key in recognizing polysaccharide antigens typically found on bacterial capsules which enhances immune response specificity.

Pathways

IgG2 acts within the adaptive immune system involving humoral immunity pathways. It participates in the classical complement activation pathway leading to the destruction of antigens through membrane attack complexes. IgG2 interacts with other immunoglobulin family members including IgG1 and IgG3 through these pathways offering a comprehensive immune response. The relationship with IgM is also notable as both IgG2 and IgM are involved in the first line of defense against polysaccharide antigens.

IgG2 levels and function relate to conditions such as recurrent bacterial infections and IgG subclass deficiencies. Patients with specific IgG2 deficiencies often suffer from increased susceptibility to infections particularly those caused by encapsulated bacteria like Streptococcus pneumoniae. Autoimmune diseases such as Systemic Lupus Erythematosus (SLE) may also involve dysfunctional IgG2 responses linking IgG2 with proteins like C1q in disease pathology. Researchers continue to study these connections to better understand immune function and improve therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors, which upon binding to a specific antigen trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs known as antibodies mediate the effector phase of humoral immunity by blocking the interaction of infectious antigens with cellular receptors (via the antigen-binding region) and eliciting effector mechanisms that lead to pathogen neutralization (via the constant region) (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The antigen-binding region is formed by the variable domain of one heavy chain paired with the variable domain of its associated light chain. Each Ig molecule has two antigen-binding sites with remarkable affinity for a particular antigen due to V-(D)-J rearrangement, somatic hypermutations and affinity maturation of the variable domains upon antigen exposure (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region defines the Ig isotype that perform distinct sets of effector functions. B cells diversify and rearrange their Ig constant regions through class-switch recombination, a process by which the constant region is switched from one Ig isotype to another, namely from IgM and IgD to IgG, IgA and IgE (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region interacts (via the fragment crystallizable, Fc) with receptors on innate immune cells and the complement system to mediate humoral effector functions. Ig gamma-2 (IgG2) isotype formed against polysaccharide structures found on encapsulated bacteria elicits antibody-dependent protection through activation of complement pathway.
See full target information IGHG2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com