Rabbit Recombinant Monoclonal IL-1 beta antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra) and reacts with Mouse, Human samples.
Images
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IL-1 beta antibody [EPR23851-127] (AB317662), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-il-1-beta-antibody-epr23851-127-ab317662--flow-cytometry-intracellular-img431843.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IL-1 beta antibody [EPR23851-127] (AB317662)")
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IL-1 beta antibody [EPR23851-127] (AB317662), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-il-1-beta-antibody-epr23851-127-ab317662--flow-cytometry-intracellular-img431842.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IL-1 beta antibody [EPR23851-127] (AB317662)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | ICC/IF | |
|---|---|---|
| Human | Tested | Not recommended |
| Mouse | Tested | Not recommended |
| Rat | Not recommended | Not recommended |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
Potent pro-inflammatory cytokine (PubMed:10653850, PubMed:12794819, PubMed:28331908, PubMed:3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed:3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33377178, PubMed:33883744). Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed:28331908).
Alternative names
IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin
Recommended products
Rabbit Recombinant Monoclonal IL-1 beta antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra) and reacts with Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR23851-127
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IL-1 beta antibody [EPR23851-127] (ab317662), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-il-1-beta-antibody-epr23851-127-ab317662--flow-cytometry-intracellular-img431843.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IL-1 beta antibody [EPR23851-127] (ab317662)")
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IL-1 beta antibody [EPR23851-127] (ab317662)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1(Human monocytic leukemia monocyte) treated with 80nM TPA for 16 hours, then 100ng/ml LPS for 3 hours and 300ng/ml BFA for another 3 hours (Red) / Untreated control (Dotted Red) cells labelling IL-1 beta with ab317662 at 1/500 dilution (0.1ug) (Red) and Dotted Red (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IL-1 beta antibody [EPR23851-127] (ab317662), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-il-1-beta-antibody-epr23851-127-ab317662--flow-cytometry-intracellular-img431842.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IL-1 beta antibody [EPR23851-127] (ab317662)")
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IL-1 beta antibody [EPR23851-127] (ab317662)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 100ng/ml LPS for 4 hours and 1ug/ml BFA for another 3 hours (Red) / Untreated control (Dotted Red) cells labelling IL-1 beta with ab317662 at 1/500 dilution (0.1ug) (Red) and Dotted Red (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com