Alexa Fluor® 488 Anti-Indoleamine 2, 3-dioxygenase antibody [EPR20374]

4 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Indoleamine 2, 3-dioxygenase antibody [EPR20374] (ab315181)

  Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling Indoleamine 2, 3-dioxygenase with ab315181 at 1/100 (5.0 ug/ml).

  Negative control: no staining on human skeletal muscle. The section was incubated with ab315181 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Indoleamine 2, 3-dioxygenase antibody [EPR20374] (ab315181)

  Immunohistochemical analysis of paraffin-embedded Human cervical cance tissue labeling Indoleamine 2, 3-dioxygenase with ab315181 at 1/100 (5.0 ug/ml).

  Positive staining on human cervical cancer. The section was incubated with ab315181 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Indoleamine 2, 3-dioxygenase antibody [EPR20374] (ab315181)

  Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Indoleamine 2, 3-dioxygenase with ab315181 at 1/100 (5.0 ug/ml).

  Positive staining on human tonsil. The section was incubated with ab315181 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Indoleamine 2, 3-dioxygenase antibody [EPR20374] (ab315181)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Indoleamine 2, 3-dioxygenase with ab315181 at 1/50 (10.0 ug/ml) dilution. Confocal image showing cytoplasmic and nuclear staining in HeLa cells treated with Human Interferon-γ (50 ng/ml) for 16 h. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).