JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB270919

Alexa Fluor® 488 Anti-Indoleamine 2, 3-dioxygenase antibody [SP260]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Indoleamine 2, 3-dioxygenase antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

IDO, INDO, IDO1, IDO-1

1 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] (AB270919)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] (AB270919)

Flow cytometry overlay histogram showing HeLa cells (green line) or HeLa cells treated with 50ng/mL IFN-gamma for 16h stained with ab270920 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab270919) (1x 106 in 100μl at 0.04μg/ml (1/12500) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor 488 ® (ab199091) used at the same concentration and conditions as the primary antibody (HeLa - black line; HeLa treated with 50ng/mL IFN-gamma for 16h - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

This antibody can also be used in cells fixed with 4% formaldehyde (10 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal

  • 578 PE

    PE Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] (C-terminal)

  • 660 APC

    APC Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] (C-terminal)

  • HRP

    HRP Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] (C-terminal)

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal

  • Unconjugated

    Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal

  • Carrier free

    Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP260

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/12500", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Indoleamine 2 3-dioxygenase (IDO) also known as IDO1 is an enzyme involved in the catabolism of tryptophan to kynurenine. It weighs approximately 45 kDa and it is an important target in immunoregulatory processes. IDO is highly expressed in antigen-presenting cells such as dendritic cells and macrophages as well as in various tumor cells where it helps modulate immune responses. This enzyme can also be measured by using assays like IDO ELISA.
Biological function summary

IDO plays a role in immune response regulation by degrading tryptophan an essential amino acid needed for T-cell proliferation. The depletion of tryptophan and the accumulation of its metabolites such as kynurenine cause immunosuppressive effects within the tissue microenvironment. While IDO functions mostly as a standalone enzyme its activity influences the cellular surroundings by altering the balance of local immune reactions.

Pathways

IDO integrates into the tryptophan metabolism pathway and is important in the kynurenine pathway. Through this pathway it maintains immune homeostasis and cell defense mechanisms. Proteins like kynureninase and kynurenine 3-monooxygenase also participate in the same metabolic processes which further extend the effects of tryptophan metabolism on immune cell behavior.

IDO is linked with cancer and chronic inflammatory conditions. In cancer elevated IDO expression suppresses anti-tumor immunity aiding tumor cells to evade immune surveillance. It also plays a role in autoimmune disorders by regulating excessive immune activity. During cancer progression IDO often works in conjunction with immune checkpoint proteins like PD-L1 which further enhances its immunosuppressive capabilities within the tumor microenvironment.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed : 17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed : 25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed : 25691885). Acts as a suppressor of anti-tumor immunity (PubMed : 14502282, PubMed : 23103127, PubMed : 25157255, PubMed : 25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed : 25691885). Protects the fetus from maternal immune rejection (PubMed : 25691885).
See full target information IDO1
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB235936

Indoleamine 2,3-Dioxygenase 1 (IDO1) Activity Assay Kit

Functional Studies - Indoleamine 2,3-Dioxygenase 1 (IDO1) Activity Assay Kit (AB235936)

  • 0
  • 0
Primary Antibodies

AB270151

Alexa Fluor® 647 Anti-SOX10 antibody [SP267]

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-SOX10 antibody [SP267] (AB270151)

  • 4
  • 1
Primary Antibodies

AB237268

Alexa Fluor® 647 Anti-Heme Oxygenase 1 antibody [EPR18161-128]

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Heme Oxygenase 1 antibody [EPR18161-128] (AB237268)

  • 0
  • 0
Primary Antibodies

AB282114

Alexa Fluor® 594 Anti-CD163 antibody [EPR19518]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD163 antibody [EPR19518] (AB282114)

  • 1
  • 1
Primary Antibodies

AB313397

Alexa Fluor® 647 Anti-CD69 antibody [EPR21814]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD69 antibody [EPR21814] (AB313397)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com