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AB275957

Alexa Fluor® 488 Anti-Integrin beta 2 antibody [IVA35]

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Mouse Monoclonal CD18 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Cow, Human samples. Immunogen corresponding to Cell preparation containing Integrin beta-2 protein.

View Alternative Names

CD18, MFI7, ITGB2, Integrin beta-2, Complement receptor C3 subunit beta

4 Images
Flow Cytometry - Alexa Fluor® 488 Anti-Integrin beta 2 antibody [IVA35] (AB275957)
  • Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 488 Anti-Integrin beta 2 antibody [IVA35] (AB275957)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab275957 (concentration in sample 5 μg/ml).

Flow Cytometry - Alexa Fluor® 488 Anti-Integrin beta 2 antibody [IVA35] (AB275957)
  • Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 488 Anti-Integrin beta 2 antibody [IVA35] (AB275957)

Separation of human neutrophil granulocytes (red-filled) from human CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab275957 (concentration in sample 5 μg/ml).

Flow Cytometry - Alexa Fluor® 488 Anti-Integrin beta 2 antibody [IVA35] (AB275957)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Anti-Integrin beta 2 antibody [IVA35] (AB275957)

Flow cytometry surface staining pattern of bovine peripheral whole blood stained using ab275957 (concentration in sample 3 μg/ml).

Flow Cytometry - Alexa Fluor® 488 Anti-Integrin beta 2 antibody [IVA35] (AB275957)
  • Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 488 Anti-Integrin beta 2 antibody [IVA35] (AB275957)

Separation of bovine leukocytes stained using ab275957 (concentration in sample 3 μg/ml, red-filled) from bovine leukocytes stained using mouse IgG1 isotype control (concentration in sample 3 μg/ml, black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

IVA35

Isotype

IgG1

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Cow, Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB315433

Human CD18 ELISA Kit

SimpleStep

Sandwich ELISA - Human CD18 ELISA Kit (AB315433)

  • 0
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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  • 20
Primary Antibodies

AB181597

Anti-Cytokeratin 18 antibody [EPR17347]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

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  • 3
Proteins & Peptides

AB153014

Recombinant Human KIAA0226 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human KIAA0226 protein (GST tag N-Terminus) (AB153014)

  • 0
  • 0
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2-5 µg/mL", "FlowCyt-species-notes": "<p></p>" }, "Cow": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2-5 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin ITGAL : ITGB2 is a receptor for ICAM1, ICAM2 and ICAM3 (PubMed : 1676048, PubMed : 23775590, PubMed : 38195629). Integrin ITGAL : ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed : 29100055). Integrins ITGAM : ITGB2 and ITGAX : ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX : ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM : ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM : ITGB2 is also a receptor for factor X. Integrin ITGAD : ITGB2 is a receptor for ICAM3 and VCAM1 (PubMed : 10438935, PubMed : 8777714, PubMed : 9841932). Contributes to natural killer cell cytotoxicity (PubMed : 15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed : 11812992, PubMed : 28807980). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed : 18587400). Integrin ITGAL : ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed : 23775590). In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407). Integrins ITGAX : ITGB2 functions as a receptor of the erythrocyte-specific adhesion molecule ICAM4 and mediates erythrophagocytosis (PubMed : 16985175). Integrins ITGAX : ITGB2 functions as a receptor of the neuron-specific adhesion molecule ICAM5 ensuring neuron cell-leukocyte adhesion (PubMed : 10741396). Integrin ITGAL : ITGB2 functions as a receptor of ICAM1 by acting as a platform at the immunological synapse to translate TCR engagement and density of the ITGAL ligand ICAM1 into graded adhesion (PubMed : 38195629). Integrin ITGAM : ITGB2/MAC-1 complex functions as a signaling receptor for the ligand receptor ICAM1, ensuring adhesion between stimulated neutrophils and stimulated endothelial cells (PubMed : 1980124). Integrin ITGAL/ITGB2 that functions as a signaling receptor of ICAM2, ensuring leukocyte cell-cell adhesion on resting cells (PubMed : 1676048).
See full target information ITGB2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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