AB222116

Alexa Fluor® 488 Anti-Keratin 12/K12 antibody [EPR17882]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal K1C12 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Cytokeratin-12, Keratin-12, CK-12, K12, KRT12

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Keratin 12/K12 antibody [EPR17882] (AB222116)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Keratin 12/K12 antibody [EPR17882] (AB222116)

ab222116 staining Keratin 12/K12 in Hek293T cells transfected with RFP-tagged Keratin 12/K12 expression construct or RFP-only-tagged expression construct. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.

The cells were then incubated overnight at +4°C with ab222116 at 1/100 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (pseudocolored in white). Nuclear DNA was labelled with DAPI (shown in blue) and RFP is shown in red.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Keratin 12/K12 antibody [EPR17882]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Keratin 12/K12 antibody [EPR17882]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17882

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in Hek293T cells transfected with RFP-tagged Keratin 12 fixed with 4% formaldehyde (10 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Keratin 12 (K12) also called keratin or keratin 1.12 is a type I cytokeratin protein with a mass of approximately 52 kDa. K12 is specifically expressed in the corneal epithelium an important part of the eye's outer structure. This expression is restricted making K12 a marker for corneal epithelial cells. Anti-keratin antibodies can help identify and visualize this protein in cytology studies.

Biological function summary

Keratin 12 is essential in maintaining the structural integrity and mechanical resilience of corneal epithelial cells. It forms part of the intermediate filament complex with other keratins providing a framework essential in cell stability and protection. The complex strengthens cell adhesion and maintains the corneal epithelium's hydration status enabling clear vision.

Pathways

The formation and maintenance of the corneal epithelium involve Keratin 12 in particular cellular signaling pathways. The protein takes part in the intermediate filament pathway interacting with proteins like Keratin 3. These interactions support the proper assembly and function of the cytoskeletal structure essential for cell shape and resilience significantly impacting corneal wound healing processes.

Keratin 12 mutations can lead to Meesmann corneal dystrophy which affects vision. This condition involves aberrant keratin filament assembly compromising corneal integrity. Keratin 3 often partners with K12 in maintaining corneal coherence and mutations affecting either protein can contribute to the disorder highlighting their interconnected roles in corneal health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in corneal epithelium organization, integrity and corneal keratin expression.

See full target information KRT12

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 48:414-430 PubMed40083774

2025

In situ UNIversal Orthogonal Network (UNION) bioink deposition for direct delivery of corneal stromal stem cells to corneal wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia G Brunel,Betty Cai,Sarah M Hull,Uiyoung Han,Thitima Wungcharoen,Gabriella Maria Fernandes-Cunha,Youngyoon Amy Seo,Patrik K Johansson,Sarah C Heilshorn,David Myung

Cancer research 83:3783-3795 PubMed37668527

2023

A Cell Surface-Binding Antibody Atlas Nominates a MUC18-Directed Antibody-Drug Conjugate for Targeting Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Shi,Tao Jiao,Qian Guo,Weining Weng,Linjie Ma,Qing Zhang,Lijun Wang,Jianjian Zhang,Caiwei Chen,Yaling Huang,Mingqiao Wang,Rong Pan,Yanfang Tang,Wenhao Hu,Tao Meng,Shu-Hui Liu,Jun Guo,Yan Kong,Xun Meng

International journal of general medicine 15:5395-5405 PubMed35685694

2022

Effects of Yunnan Baiyao on the Differentiation of HPDLFs on the Bio-Oss Collagen Scaffold in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Yu,Jing Wang,Qianqian Han,Wen Chu,Shaowen Lu,Yu Liu,Yi Peng,Jie Xu,Yanqing Shui

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-Keratin 12/K12 antibody [EPR17882]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Keratin 12/K12 antibody [EPR17882] (AB222116)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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