Alexa Fluor® 488 Anti-Ki67 antibody [SP6]

4 product images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Ki67 antibody [SP6] (ab281847)

  Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Ki67 with ab281847.

  Positive staining on human colon. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab281847 at 1/100 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Ki67 antibody [SP6] (ab281847)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling Ki67 with ab281847 at 1/50 dilution (10.0 µg/ml) (Green).

  Confocal image showing nucleolar staining in HeLa cells. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

• 

  Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Ki67 antibody [SP6] (ab281847)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma cell) cells labelling Ki67 with ab281847 at 1/5000 dilution (0.01µg) (Right) compared with a Rabbit monoclonal isotype control - Alexa Fluor^® 488 (Left) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  Cells were co-stained with DRAQ5 to differentiate cell cycle phase.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Ki67 antibody [SP6] (ab281847)

  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Ki67 with ab281847.

  Positive staining on mouse colon. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab281847 at 1/100 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)