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AB281847

Alexa Fluor® 488 Anti-Ki67 antibody [SP6]

5

(1 Review)

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(11 Publications)

Anti-Ki67 antibody [SP6] - Alexa Fluor® 488 conjugated (ab281847) is a rabbit recombinant monoclonal antibody detecting Ki67 in Flow Cytometry (Intra), IHC-P, ICC/IF. Suitable for Human, Mouse.

- Clone SP6 is the most cited clone to Ki67
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

4 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Ki67 antibody [SP6] (AB281847)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Ki67 antibody [SP6] (AB281847)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma cell) cells labelling Ki67 with ab281847 at 1/5000 dilution (0.01μg) (Right) compared with a Rabbit monoclonal isotype control - Alexa Fluor® 488 (Left) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Cells were co-stained with DRAQ5 to differentiate cell cycle phase.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Ki67 antibody [SP6] (AB281847)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Ki67 antibody [SP6] (AB281847)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling Ki67 with ab281847 at 1/50 dilution (10.0 μg/ml) (Green).

Confocal image showing nucleolar staining in HeLa cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Ki67 antibody [SP6] (AB281847)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Ki67 antibody [SP6] (AB281847)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Ki67 with ab281847.

Positive staining on human colon. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab281847 at 1/100 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Ki67 antibody [SP6] (AB281847)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Ki67 antibody [SP6] (AB281847)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Ki67 with ab281847.

Positive staining on mouse colon. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab281847 at 1/100 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP6

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse, Human, Human, Mouse, Rat

Applications

ICC/IF, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-Ki67 antibody [SP6] (ab281847) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

Collaboration
Anti-Ki67 [SP6] Alexa Fluor® 488 (ab281847) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Related products
Antibody clone SP6 is also available pre-conjugated to a variety of labels for your convenience – Anti-Ki67 Alexa Fluor® 488 [SP6] (ab281847).

Other related products
We have a range of other formats of antibody clone [SP6] also available for your convenience: ab16667, ab21700, Carrier free - ab197547, ab231172, ab238624, Oligonucleotide - ab279361, Alexa Fluor® 488 - ab281847, Alexa Fluor® 647 - ab281928, PE - ab282173, APC - ab314285, ab320709, Carrier free - ab320710, ab323292

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ki67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.
Biological function summary

The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.

Pathways

Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.

Ki67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein that associates with the surface of mitotic chromosomes and acts both as a chromosome repellent during early mitosis and chromosome attractant during late mitosis (PubMed : 27362226, PubMed : 32879492, PubMed : 35513709, PubMed : 39153474). Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed : 27362226). During early mitosis, relocalizes from nucleoli to the chromosome surface where it forms extended brush structures that cover a substantial fraction of the chromosome surface (PubMed : 27362226). The MKI67 brush structure prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier : the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed : 27362226). During mitotic anaphase, the MKI67 brush structure collapses and MKI67 switches from a chromosome repellent to a chromosome attractant to promote chromosome clustering and facilitate the exclusion of large cytoplasmic particles from the future nuclear space (PubMed : 32879492, PubMed : 39153474). Mechanistically, dephosphorylation during mitotic exit and simultaneous exposure of a conserved basic patch induce the RNA-dependent formation of a liquid-like condensed phase on the chromosome surface, promoting coalescence of neighboring chromosome surfaces and clustering of chromosomes (PubMed : 39153474). Binds premature ribosomal RNAs during anaphase; promoting liquid-liquid phase separation (PubMed : 28935370, PubMed : 39153474). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed : 10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization; it is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in mitotic chromosome (PubMed : 24867636).
See full target information MKI67
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1611637 PubMed40693271

2025

Antagonizing IL-6 receptor restores pancreatic tissue resident NK cells activation and ameliorates pancreatic injury in the mouse model of MASH.

Applications

Unspecified application

Species

Unspecified reactive species

Johnny Amer,Diana Abu Arra,Ahmad Salhab,Faris Kayed,Muneer Maali,Raghad Shweiki,Mustafa Ghanim

Drug design, development and therapy 19:2677-2691 PubMed40226128

2025

Huyang Yangkun Formula Enhances Ovarian Function and Delays Reproductive Aging by Influencing Hypothalamic GnRH/LH Pulse Release Through GAT-1/GABA/GABAAR2.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Yin Peng,Guangning Nie,Fangping Cheng,Yuling Zhou,Jian Liu,Hongyan Yang

Bioactive materials 48:414-430 PubMed40083774

2025

In situ UNIversal Orthogonal Network (UNION) bioink deposition for direct delivery of corneal stromal stem cells to corneal wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia G Brunel,Betty Cai,Sarah M Hull,Uiyoung Han,Thitima Wungcharoen,Gabriella Maria Fernandes-Cunha,Youngyoon Amy Seo,Patrik K Johansson,Sarah C Heilshorn,David Myung

Communications biology 8:296 PubMed40033126

2025

Maternal immune activation followed by peripubertal stress combinedly produce reactive microglia and confine cerebellar cognition.

Applications

Unspecified application

Species

Unspecified reactive species

Momoka Hikosaka,Md Sorwer Alam Parvez,Yuki Yamawaki,Souichi Oe,Yuan Liang,Yayoi Wada,Yukie Hirahara,Taro Koike,Hirohiko Imai,Naoya Oishi,Sina M Schalbetter,Asuka Kumagai,Mari Yoshida,Takeshi Sakurai,Masaaki Kitada,Urs Meyer,Shuh Narumiya,Gen Ohtsuki

Molecular medicine (Cambridge, Mass.) 31:37 PubMed39901071

2025

Conventional type 1 dendritic cells in the lymph nodes aggravate neuroinflammation after spinal cord injury by promoting CD8 T cell expansion.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Qing Wang,Xiao-Yi Wang,Yue-Hui Ma,Heng-Jun Zhou

Nature immunology 25:2152-2165 PubMed39438660

2024

A longitudinal single-cell atlas of anti-tumour necrosis factor treatment in inflammatory bowel disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Thomas,Matthias Friedrich,Charlotte Rich-Griffin,Mathilde Pohin,Devika Agarwal,Julia Pakpoor,Carl Lee,Ruchi Tandon,Aniko Rendek,Dominik Aschenbrenner,Ashwin Jainarayanan,Alexandru Voda,Jacqueline H Y Siu,Raphael Sanches-Peres,Eloise Nee,Dharshan Sathananthan,Dylan Kotliar,Peter Todd,Maria Kiourlappou,Lisa Gartner,Nicholas Ilott,Fadi Issa,Joanna Hester,Jason Turner,Saba Nayar,Jonas Mackerodt,Fan Zhang,Anna Jonsson,Michael Brenner,Soumya Raychaudhuri,Ruth Kulicke,Danielle Ramsdell,Nicolas Stransky,Ray Pagliarini,Piotr Bielecki,Noah Spies,Brian Marsden,Stephen Taylor,Allon Wagner,Paul Klenerman,Alissa Walsh,Mark Coles,Luke Jostins-Dean,Fiona M Powrie,Andrew Filer,Simon Travis,Holm H Uhlig,Calliope A Dendrou,Christopher D Buckley

Frontiers in veterinary science 11:1385395 PubMed38725585

2024

Impact of platelet lysate on immunoregulatory characteristics of equine mesenchymal stromal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Moellerberndt,Sabine Niebert,Kerstin Fey,Alina Hagen,Janina Burk

Archiv der Pharmazie 357:e2400063 PubMed38704748

2024

Low exposition to lithium prevents nephrogenic diabetes insipidus but not microcystic dilations of the collecting ducts in long-term rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Nahid Tabibzadeh,Mathieu Klein,Mélanie Try,Joël Poupon,Pascal Houillier,Christophe Klein,Lydie Cheval,Gilles Crambert,Samia Lasaad,Lucie Chevillard,Bruno Megarbane

Frontiers in veterinary science 10:1154987 PubMed37346276

2023

Influence of Rho/ROCK inhibitor Y-27632 on proliferation of equine mesenchymal stromal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Michaela Melzer,Janina Burk,Deborah J Guest,Jayesh Dudhia

Computational and mathematical methods in medicine 2022:8388473 PubMed35664641

2022

Nanohydroxyapatite Hydrogel Can Promote the Proliferation and Migration of Chondrocytes and Better Repair Talar Articular Cartilage.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxuan Zhang,Yi Cui,Jian Tian,Xueming Chen,Tonglong Xu,Jiajia Liu,Yajun Xu
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Product promise

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