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AB313349

Alexa Fluor® 488 Anti-KLF4 antibody [EPR20753-25]

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Rabbit Recombinant Monoclonal KLF4 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Mouse, Rat samples.

View Alternative Names

Ezf, Gklf, Zie, Klf4, Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-KLF4 antibody [EPR20753-25] (AB313349)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-KLF4 antibody [EPR20753-25] (AB313349)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling KLF4 with ab313349 at 1/100 (5.0 ug/ml). Nuclear staining on mouse colon. The section was incubated with ab313349 for 60 mins at room temperature (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-KLF4 antibody [EPR20753-25] (AB313349)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-KLF4 antibody [EPR20753-25] (AB313349)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labelling KLF4 with ab313349 at 1/100 (5.0 ug/ml). Nuclear staining on rat colon. The section was incubated with ab313349 for 60 mins at room temperature (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20753-25

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Rat, Mouse

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLF4 also known as Kruppel-like factor 4 acts as a transcription factor involved in the regulation of gene expression. It has a molecular weight of approximately 55-60 kDa. Researchers often find KLF4 expressed in epithelial tissues such as the skin and gastrointestinal tract. It regulates cellular processes like differentiation proliferation and apoptosis playing a role in maintaining homeostasis and cell identity.
Biological function summary

KLF4 impacts cell differentiation and proliferation through its role as a transcription factor. It functions within several cellular complexes and interacts with other transcription factors to modulate gene expression. KLF4 regulates stem cell maintenance by influencing gene networks involved in self-renewal and differentiation contributing particularly to pluripotency in embryonic stem cells.

Pathways

The transcriptional activity of KLF4 integrates into key signaling pathways like the Wnt/β-catenin and Notch pathways. Within the Wnt/β-catenin pathway KLF4 may cooperate with β-catenin and TCF/LEF transcription factors to modulate gene targets. Similarly in the Notch signaling pathway KLF4 interacts with pathway components to influence cell fate decisions. These interactions highlight KLF4's central role in orchestrating complex signaling networks.

Mutations or dysregulated expression of KLF4 associates with various conditions including colorectal cancer and skin disorders like atopic dermatitis. In colorectal cancer KLF4 often interacts with other oncogenes and tumor suppressor proteins such as p53 to influence tumorigenesis. In skin disorders altered KLF4 expression can impact skin barrier function and inflammatory responses connecting it with proteins involved in immune response regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence (PubMed : 10431239, PubMed : 10556311, PubMed : 15358627, PubMed : 16954384, PubMed : 17060454, PubMed : 19816951, PubMed : 20071344, PubMed : 29593216). Binds to the promoter region of its own gene and can activate its own transcription (PubMed : 10431239, PubMed : 10556311, PubMed : 15358627, PubMed : 16954384, PubMed : 17060454, PubMed : 19816951, PubMed : 20071344, PubMed : 29593216). Regulates the expression of key transcription factors during embryonic development (PubMed : 10431239, PubMed : 10556311, PubMed : 15358627, PubMed : 16954384, PubMed : 17060454, PubMed : 19816951, PubMed : 20071344, PubMed : 29593216). Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation (PubMed : 10431239, PubMed : 10556311, PubMed : 15358627, PubMed : 16954384, PubMed : 17060454, PubMed : 19816951, PubMed : 20071344, PubMed : 29593216). Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription (By similarity).
See full target information Klf4
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Product promise

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For full details, please see our Terms & Conditions

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