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Rabbit Recombinant Monoclonal Klotho antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Klotho antibody [EPR6856] (AB311042), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Klotho antibody [EPR6856] (AB311042), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 488 Anti-Klotho antibody [EPR6856] (AB311042), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 488 Anti-Klotho antibody [EPR6856] (AB311042), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity). The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

Alternative names

Klotho, KL

Recommended products

Rabbit Recombinant Monoclonal Klotho antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR6856
Purification technique
Affinity purification Protein A
Specificity

When we tested the antibody in human samples, the only form of the protein that we are able to detect is the secreted one (about 65kDa). When we tested the antibody in mouse samples (wt vs KO) we could only detect the membrane bound form of the protein (about 130kDa). This difference could be due to the nature of the samples (different species). However, we do not have a clear explanation about this. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented here.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Klotho also known as alpha Klotho protein is a multifunctional protein prominently involved in regulating aging processes. It consists of approximately 130 kDa and is expressed mainly in the kidneys brain and parathyroid gland. This membrane-bound protein may also exist in a soluble form after cleavage by specific enzymes. Alpha Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) facilitating phosphate and vitamin D metabolism regulation.

Biological function summary

Alpha Klotho influences several cellular processes such as aging and mineral ion homeostasis. As a part of the FGF23-Klotho complex it enables the fibroblast growth factor to exert its biological effects. This protein modulates the effects of FGF23 on the renal excretion of phosphate and the synthesis of vitamin D. By interacting with FGF23 alpha Klotho supports maintaining electrolyte balance vital for proper physiological functioning.

Pathways

The FGF23-Klotho axis integrates into significant signaling pathways regulating mineral metabolism and aging. Klotho protein interactions with FGF23 contribute to controlling phosphate homeostasis by inhibiting renal phosphate reabsorption and suppressing vitamin D synthesis. It also engages with other proteins notably the fibroblast growth factors to regulate aging-related pathways ensuring cellular longevity and repair mechanisms are optimized.

Associated diseases and disorders

Understanding Klotho's role helps in managing conditions like chronic kidney disease and some neurological disorders. Research indicates that reduced levels of alpha Klotho may correspond to kidney dysfunction due to its critical role in mineral metabolism. Furthermore alterations in Klotho expression connect with neurodegenerative diseases implicating its interaction with other proteins such as amyloid precursor protein and tau which are significant in disorders like Alzheimer's disease.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Klotho antibody [EPR6856] (ab311042), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Klotho antibody [EPR6856] (ab311042)

    Immunofluorescence staining of Klotho staining in a section of formalin-fixed paraffin-embedded human kidney normal*.

    Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab311042 at 1/100 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Klotho antibody [EPR6856] (ab311042), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Klotho antibody [EPR6856] (ab311042)

    Negative immunofluorescence staining of Klotho staining in a section of formalin-fixed paraffin-embedded human skeletal muscle*.

    Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab311042 at 1/100 dilution. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Flow Cytometry - Alexa Fluor® 488 Anti-Klotho antibody [EPR6856] (ab311042), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Anti-Klotho antibody [EPR6856] (ab311042)

    Negative immunofluorescence staining of Klotho staining in a section of formalin-fixed paraffin-embedded human skeletal muscle*.

    Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab311042 at 1/100 dilution. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Flow Cytometry - Alexa Fluor® 488 Anti-Klotho antibody [EPR6856] (ab311042), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Anti-Klotho antibody [EPR6856] (ab311042)

    Immunofluorescence staining of Klotho staining in a section of formalin-fixed paraffin-embedded human kidney normal*.

    Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab311042 at 1/100 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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