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AB317297

Alexa Fluor® 488 Anti-Klra8 antibody [EPR28281-49]

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Rabbit Recombinant Monoclonal KLRA8 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

Ly-49h, Ly49-h, Ly49H, Klra8, Killer cell lectin-like receptor 8, Lymphocyte antigen 49h, T-cell surface glycoprotein Ly-49H

2 Images
Flow Cytometry - Alexa Fluor® 488 Anti-Klra8 antibody [EPR28281-49] (AB317297)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Anti-Klra8 antibody [EPR28281-49] (AB317297)

Flow cytometric analysis of mouse splenocytes cells labelling Klra8 with ab317297 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control.

Mouse splenocytes are co-stained with NK1.1 conjugated Alexa Fluor®647. Gated on viable cell.

Flow Cytometry - Alexa Fluor® 488 Anti-Klra8 antibody [EPR28281-49] (AB317297)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Anti-Klra8 antibody [EPR28281-49] (AB317297)

Flow cytometric analysis of mouse splenocytes cells labelling Klra8 with ab317297 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control.

Mouse splenocytes are co-stained with CD3 conjugated Alexa Fluor®647. Gated on viable cell.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28281-49

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Klra8 also known as Ly49H is a C-type lectin-like receptor found primarily on the surface of natural killer (NK) cells. It plays a significant role in immune surveillance against viral infections. The mass of Klra8 in its protein form is approximately 44 kDa. Expression of Klra8 is mainly in NK cells although some expression can also be observed in other immune cells under specific conditions.
Biological function summary

Natural killer cells utilize Klra8 to detect and destroy infected or transformed host cells. Klra8 plays an important role in recognizing ligands expressed by pathogens specifically certain strains of the mouse cytomegalovirus (MCMV). It is not a part of a larger protein complex. This receptor directly contributes to the cell-mediated cytotoxic response by NK cells facilitating the rapid elimination of threats to the host organism.

Pathways

Klra8 operates within the immune response pathways critical for defense against viral infections. It interacts closely with components of innate immune signaling. Related proteins include DAP12 an adaptor protein that transmits activation signals from Klra8 leading to NK cell activation and subsequent target cell lysis. This pathway represents an important line of defense in the innate immune system.

Klra8's role is particularly noted in the context of viral infections such as those caused by cytomegalovirus. Deficiencies or alterations in Klra8 expression have implications in viral susceptibility and immune regulation disorders. The receptor's connection to proteins such as DAP12 links it to other immune-mediated conditions where disruptions in NK cell function may result in increased vulnerability to viral pathogens or affect immune homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor on natural killer (NK) cells for class I MHC.
See full target information Klra8
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com