Skip to main content

Rabbit Recombinant Monoclonal L1CAM antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Flow Cytometry - Alexa Fluor® 488 Anti-L1CAM antibody [EPR23241-224] (AB275189), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-L1CAM antibody [EPR23241-224] (AB275189), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68.98% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.

Alternative names

Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1, L1CAM, CAML1, MIC5

Recommended products

Rabbit Recombinant Monoclonal L1CAM antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23241-224

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - Alexa Fluor® 488 Anti-L1CAM antibody [EPR23241-224] (ab275189), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Anti-L1CAM antibody [EPR23241-224] (ab275189)

    Flow cytometry overlay histogram showing right HeLa positive cells and left negative L1CAM knockout HeLa cells (Human L1CAM knockout HeLa cell line ab255401) cells stained with ab275189 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab275189) (1x 106 in 100μl at 10μg/ml (1/50)) for 30 min on ice. Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor 488® (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. 5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-L1CAM antibody [EPR23241-224] (ab275189), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-L1CAM antibody [EPR23241-224] (ab275189)

    ab275189 staining L1CAM in wild-type HeLa cells, with negative expression in L1CAM knockout HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab275189 at 1 μg/ml (shown in Green). Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647) (shown in red) was used at 2 μg/ml for structural counterstaining.

    Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

    This product also work with 4% formaldehyde (10 min) fixation under the same testing conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com