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Rabbit Recombinant Monoclonal LAMA1 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-LAMA1 antibody [EPR27258-37] (AB317065), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-LAMA1 antibody [EPR27258-37] (AB317065), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-LAMA1 antibody [EPR27258-37] (AB317065), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-LAMA1 antibody [EPR27258-37] (AB317065), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Mouse
Tested
Rat
Tested

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Alternative names

Lama, Lama-1, Lama, Lama-1, Lama1, Laminin subunit alpha-1, Laminin A chain, Laminin-1 subunit alpha, Laminin-3 subunit alpha, S-laminin subunit alpha, S-LAM alpha

Rabbit Recombinant Monoclonal LAMA1 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Mouse, Rat samples.

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR27258-37

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

LAMA1 also known as laminin subunit alpha-1 is a component of the extracellular matrix with a molecular mass of approximately 400 kDa. It is part of the laminin family a group of glycoproteins that play key roles in maintaining structural integrity and signaling in tissues. LAMA1 is widely expressed in basement membranes of many tissues particularly in the brain kidney and other organs with complex architecture. Researchers often explore its expression patterns using techniques like laminin histology and laminin microscope analysis.

Biological function summary

This protein acts as a structural scaffold and cell adhesion mediator. LAMA1 integrates into the laminin-1 trimer complex composed of alpha beta and gamma chains supporting cellular attachment differentiation and migration. This assembly aids in tissue morphogenesis and repair processes. It creates an important interface between cells and the extracellular matrix influencing cellular behavior and maintaining tissue function.

Pathways

LAMA1 interacts with cellular signaling cascades such as the PI3K/AKT pathway and the TGF-beta pathway. These pathways play significant roles in cellular growth survival and differentiation. LAMA1's interaction with other proteins like integrins and heparan sulfate proteoglycans facilitates its role in these pathways impacting diverse processes like embryonic development and tissue remodeling.

Associated diseases and disorders

LAMA1 shows a connection to conditions such as Pierson syndrome and certain congenital muscular dystrophies. Mutations in LAMA1 can lead to basement membrane defects contributing to these diseases' pathological features. Additionally interactions with proteins like collagen IV and perlecan are significant as these complexes can further influence disease severity and progression. Understanding LAMA1's role provides insights into potential therapeutic approaches for related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-LAMA1 antibody [EPR27258-37] (ab317065), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-LAMA1 antibody [EPR27258-37] (ab317065)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling LAMA1 with ab317065 at 1/50 (10.0 ug/ml) dilution.

    Negative control: no staining on mouse colon. The section was incubated with ab317065 at 4°C overnight (shown in green), followed by an Autofluorescence blocker Kit according to its datasheet. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-LAMA1 antibody [EPR27258-37] (ab317065), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-LAMA1 antibody [EPR27258-37] (ab317065)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling LAMA1 with ab317065 at 1/50 (10.0 ug/ml) dilution.

    Basement membranous staining on rat kidney. The section was incubated with ab317065 at 4°C overnight (shown in green), followed by an Autofluorescence blocker Kit according to its datasheet. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-LAMA1 antibody [EPR27258-37] (ab317065), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-LAMA1 antibody [EPR27258-37] (ab317065)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling LAMA1 with ab317065 at 1/50 (10.0 ug/ml) dilution.

    Basement membranous staining on rat testis. The section was incubated with ab317065 at 4°C overnight (shown in green), followed by an Autofluorescence blocker Kit according to its datasheet. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-LAMA1 antibody [EPR27258-37] (ab317065), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-LAMA1 antibody [EPR27258-37] (ab317065)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling LAMA1 with ab317065 at 1/50 (10.0 ug/ml) dilution.

    Basement membranous staining on mouse testis. The section was incubated with ab317065 at 4°C overnight (shown in green), followed by an Autofluorescence blocker Kit according to its datasheet. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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