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Rabbit Recombinant Monoclonal LEPRE1/P3H1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (AB223430), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (AB223430), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt (Intra)
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

1 product for Alternative Version

Target data

Function

Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts.

Alternative names

GROS1, LEPRE1, PSEC0109, P3H1, Prolyl 3-hydroxylase 1, Growth suppressor 1, Leucine- and proline-enriched proteoglycan 1, Leprecan-1

Recommended products

Rabbit Recombinant Monoclonal LEPRE1/P3H1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR10193(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LEPRE1 also known as P3H1 or prolyl 3-hydroxylase 1 is an enzyme involved in collagen synthesis with a molecular mass of approximately 71 kDa. It is a member of the prolyl hydroxylase family and carries out 3-hydroxylation of proline residues in collagen. The enzyme localizes largely to the endoplasmic reticulum where it contributes to the post-translational modification of collagen. LEPRE1 is ubiquitously expressed with higher concentrations observed in tissues rich in collagen such as skin bone and cartilage.

Biological function summary

LEPRE1 plays a fundamental role in the maturation of collagen which is an important structural protein in connective tissues. This enzyme functions as a part of the collagen prolyl 3-hydroxylation complex which includes cartilage-associated protein (CRTAP) and cyclophilin B (PPIB). The complex is responsible for the correct folding and stability of collagen molecules ensuring the proper formation of the extracellular matrix. This structural role is essential for maintaining tissue integrity and function.

Pathways

LEPRE1 is important in the collagen biosynthesis and modifying pathways. It interacts with other biosynthesis enzymes like lysyl oxidase and procollagen-proline dioxygenase. These proteins coordinate to execute post-translational modifications which stabilize the collagen triple helix structure. Pirh2 and Hsp47 act along with LEPRE1 in similar pathways helping in folding and secretion of collagen.

Associated diseases and disorders

LEPRE1 mutations have been linked to connective tissue disorders most notably osteogenesis imperfecta a genetic disorder causing brittle bones. Such mutations impair collagen modification leading to defective collagen formation and bone fragility. Additionally dysregulation of LEPRE1 activity appears connected to disorder Ehlers-Danlos syndrome noted for joint hypermobility and skin elasticity issues. COL1A1 and COL1A2 two primary components of type I collagen often associate with LEPRE1 in disease contexts as mutations in these collagens lead to similar clinical manifestations.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab223430), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab223430)

    Overlay histogram showing HeLa cells stained with ab223430 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223430, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 488 (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab223430), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab223430)

    ab223430 staining LEPRE1/P3H1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223430 at 1/1000 dilution (shown in Green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com