AB206079

Alexa Fluor® 488 Anti-Lin28A antibody [EPR4640]

RabMAb
Recombinant
What is this?

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(1 Publication)

Anti-Lin28A antibody [EPR4640] - Alexa Fluor® 488 conjugated (ab206079) is a rabbit recombinant monoclonal antibody detecting Lin28A in Flow Cytometry, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CSDD1, LIN28, ZCCHC1, LIN28A, Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lin28A antibody [EPR4640] (AB206079)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lin28A antibody [EPR4640] (AB206079)

ab206079 staining Lin28A in Jeg-3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206079 at a 1/250 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in Jeg-3 cells fixed with 100% methanol (5 min)

Flow Cytometry - Alexa Fluor® 488 Anti-Lin28A antibody [EPR4640] (AB206079)

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Anti-Lin28A antibody [EPR4640] (AB206079)

Flow Cytometry analysis of HeLa (human cervical adenocarcinoma epithelial cell, Left) and NCCIT (human pluripotent embryonic carcinoma epithelial cell, Right) labeling Lin28A with ab206079 at 1/5000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. ab199091 was used as isotype control (black). Unlabelled control/colour : Cells without incubation with primary antibody and secondary antibody (blue). Negative control : HeLa.

578 PE

PE Anti-Lin28A antibody [EPR4640]
660 APC

APC Anti-Lin28A antibody [EPR4640]
HRP

HRP Anti-Lin28A antibody [EPR4640]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Lin28A antibody [EPR4640]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Lin28A antibody [EPR4640]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Lin28A antibody [EPR4640]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Lin28A antibody [EPR4640]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4640

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in Jeg-3 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)</p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/5000", "FlowCyt-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-Lin28A antibody [EPR4640] (ab206079) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EPR4640 is also available pre-conjugated to a variety of labels for your convenience – Anti-Lin28A Alexa Fluor® 488 [EPR4640] (ab206079).

Other related products
We have a range of other formats of antibody clone [EPR4640] also available for your convenience: ab124765, Alexa Fluor® 488 - ab206079, Carrier free - ab226089, PE - ab303110, APC - ab303111, HRP - ab303112, Alexa Fluor® 647 - ab307629, Alkaline Phosphatase - ab308795, Alexa Fluor® 594 - ab310511, Alexa Fluor® 555 - ab312040, Alexa Fluor® 568 - ab312518

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism (PubMed : 21247876). Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds the miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits TUT4 and TUT7 uridylyltransferases (PubMed : 18951094, PubMed : 19703396, PubMed : 22118463, PubMed : 22898984). This results in the terminal uridylation of target pre-miRNAs (PubMed : 18951094, PubMed : 19703396, PubMed : 22118463, PubMed : 22898984). Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells (PubMed : 18951094, PubMed : 19703396, PubMed : 22118463, PubMed : 22898984). Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Binds to and enhances the translation of mRNAs for several metabolic enzymes, such as PFKP, PDHA1 or SDHA, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue (By similarity).

See full target information LIN28A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2019:2945083 PubMed31885495

2019

Tumor Necrosis Factor (TNF) Receptor Expression Determines Keratinocyte Fate upon Stimulation with TNF-Like Weak Inducer of Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuening Wang,Dan Cheng,Guanglei Hu,Lili Liang,Fei Tan,Tong Xiao,Shengxiang Xiao,Yumin Xia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-Lin28A antibody [EPR4640]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lin28A antibody [EPR4640] (AB206079)

Flow Cytometry - Alexa Fluor® 488 Anti-Lin28A antibody [EPR4640] (AB206079)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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