JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB206723

Alexa Fluor® 488 Anti-Lipoamide Dehydrogenase antibody [EPR6635]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal Lipoamide Dehydrogenase antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

GCSL, LAD, PHE3, DLD, Dihydrolipoamide dehydrogenase, Glycine cleavage system L protein

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lipoamide Dehydrogenase antibody [EPR6635] (AB206723)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lipoamide Dehydrogenase antibody [EPR6635] (AB206723)

ab206723 staining Lipoamide Dehydrogenase in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206723 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in MCF7 cells fixed with 100% methanol (5 min).

  • Carrier free

    Anti-Lipoamide Dehydrogenase antibody [EPR6635] - BSA and Azide free

  • Unconjugated

    Anti-Lipoamide Dehydrogenase antibody [EPR6635]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6635

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB226202

Anti-Lipoamide Dehydrogenase antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoamide Dehydrogenase antibody - C-terminal (AB226202)

  • 0
  • 0
Primary Antibodies

AB124926

Anti-Lipoamide Dehydrogenase antibody [EPR6634(B)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoamide Dehydrogenase antibody [EPR6634(B)] (AB124926)

  • 0
  • 0

Complementary Products

Assay Kits

AB83431

Alpha Ketoglutarate (alpha KG) Assay Kit

Functional Studies - Alpha Ketoglutarate (alpha KG) Assay Kit (AB83431)

  • 0
  • 0
Primary Antibodies

AB250066

Anti-DLST antibody [EPR13319(B)] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLST antibody [EPR13319(B)] - BSA and Azide free (AB250066)

  • 0
  • 0
Proteins & Peptides

AB152338

Recombinant Human DLST protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human DLST protein (GST tag N-Terminus) (AB152338)

  • 0
  • 0
Cell Lines & Lysates

AB265535

Human DLD (Lipoamide Dehydrogenase) knockout HeLa cell line

Advanced Validation

Western blot - Human DLD (Lipoamide Dehydrogenase) knockout HeLa cell line (AB265535)

  • 0
  • 0
Primary Antibodies

AB172617

Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (AB172617)

  • 4
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in MCF7 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex) (PubMed : 15712224, PubMed : 16442803, PubMed : 16770810, PubMed : 17404228, PubMed : 20160912, PubMed : 20385101). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion (PubMed : 29211711). A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones : associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (PubMed : 29211711). In monomeric form may have additional moonlighting function as serine protease (PubMed : 17404228). Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction (By similarity). The pyruvate dehydrogenase (PDH) complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle (Probable). It contains multiple copies of three enzymatic components : pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3) (Probable). The E3 subunit catalyzes reoxidation of the dihydrolipoyl moiety on lipoyl-bearing domains (LBDs) of E2 with NAD+ as the ultimate electron acceptor (PubMed : 16442803, PubMed : 16770810, PubMed : 20160912, PubMed : 20385101).
See full target information DLD
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 8: PubMed31309927

2019

Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Kh Stavoe,Pallavi P Gopal,Andrea Gubas,Sharon A Tooze,Erika Lf Holzbaur
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com