AB283276

Alexa Fluor® 488 Anti-Ly6g antibody [EPR22909-135]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Ly6g antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g

2 Images

Flow Cytometry - Alexa Fluor® 488 Anti-Ly6g antibody [EPR22909-135] (AB283276)

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Anti-Ly6g antibody [EPR22909-135] (AB283276)

Flow cytometric analysis of HEK-293T (Human embryonic kidney epithelial cell) transfected with HA-tagged Ly6c overexpression construct (Left) HEK-293T transfected with HA-tagged Ly6g overexpression construct (Right) cells labelling Ly6g with ab283276 at 1/500 dilution (0.1μg) compared with a isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Ly6c (Left) and Ly6g (Right) overexpression cells were surface stained with ab283276 and anti-HA tag conjugated to Alexa Fluor^®647.

ab283276 does not cross-react with Ly6c.

Gated on viable cells.

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Anti-Ly6g antibody [EPR22909-135] (AB283276)

Flow cytometric analysis of mouse blood cells cells labelling Ly6g with ab283276 at 1/50 dilution (1 μg) (Right) compared with a Rabbit monoclonal isotype control - Alexa Fluor^® 488 (Left) isotype control.

Cells were stained with rabbit IgG or ab283276, then stained with anti-CD11b conjugated to BV421.

Gated on viable cells.

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135]
578 PE

PE Anti-Ly6g antibody [EPR22909-135]
660 APC

APC Anti-Ly6g antibody [EPR22909-135]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Ly6g antibody [EPR22909-135]
519 FITC

FITC Anti-Ly6g antibody [EPR22909-135]
Unconjugated

Anti-Ly6g antibody [EPR22909-135]
Carrier free

Anti-Ly6g antibody [EPR22909-135] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22909-135

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ly6G also known as lymphocyte antigen 6 complex locus G6D 1A8 or 8C5 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein. The molecule has a molecular weight of about 25-30 kDa. Its expression is mainly on neutrophils and a subpopulation of eosinophils in mice. It plays a role in the innate immune system marking these immune cells for differentiation and response to infection. Researchers consider it a specific marker for detecting and isolating mouse neutrophils often using methods such as Ly6G staining or anti-Ly6G antibodies for identification.

Biological function summary

Ly6G functions in the immune response by facilitating the migration and adhesion of neutrophils to the site of infection or injury. It is part of a protein complex on the cell surface that includes other Ly6 proteins. This group of proteins helps to modulate signal transduction for immune cell functions. Ly6G serves as an anchor through its GPI linkage aiding cell-to-cell communication and signal amplification in inflammation processes.

Pathways

Ly6G is significantly integrated into the immune signaling pathways notably the toll-like receptor (TLR) signaling pathway. Through these pathways Ly6G influences neutrophil migration and activation. Closely associated proteins include CD11b and CD18 which interact with Ly6G in neutrophil functions including adhesion and transmigration. These pathways are essential for orchestrating the body's defense mechanisms against pathogens.

Ly6G plays an essential role in inflammatory conditions and autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of Ly6G can alter neutrophil behavior which contributes to disease pathology. Researchers are investigating the relationship between Ly6G and other immune-related proteins like myeloperoxidase (MPO) and elastase as these protein interactions may impact the development and progression of inflammatory diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ly6g

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:941219 PubMed35911675

2022

Cold Plasma Irradiation Attenuates Atopic Dermatitis Enhancing HIF-1α-Induced MANF Transcription Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Sun,Xinru Zhang,Chao Hou,Shujun Yu,Yujing Zhang,Zhuo Yu,Ling Kong,Changqing Liu,Lijie Feng,Dong Wang,Guohua Ni

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-Ly6g antibody [EPR22909-135]

Flow Cytometry - Alexa Fluor® 488 Anti-Ly6g antibody [EPR22909-135] (AB283276)

Flow Cytometry - Alexa Fluor® 488 Anti-Ly6g antibody [EPR22909-135] (AB283276)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise