Rabbit Recombinant Monoclonal Ly6g antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.
IgG
Rabbit
Alexa Fluor® 488
Ex: 495nm, Em: 519nm
pH: 7.2 - 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
Flow Cyt | |
---|---|
Mouse | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
Select an associated product type
Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g
Rabbit Recombinant Monoclonal Ly6g antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.
IgG
Rabbit
Alexa Fluor® 488
Ex: 495nm, Em: 519nm
pH: 7.2 - 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
EPR22909-135
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Store in the dark
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
This supplementary information is collated from multiple sources and compiled automatically.
Ly6G also known as lymphocyte antigen 6 complex locus G6D 1A8 or 8C5 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein. The molecule has a molecular weight of about 25-30 kDa. Its expression is mainly on neutrophils and a subpopulation of eosinophils in mice. It plays a role in the innate immune system marking these immune cells for differentiation and response to infection. Researchers consider it a specific marker for detecting and isolating mouse neutrophils often using methods such as Ly6G staining or anti-Ly6G antibodies for identification.
Ly6G functions in the immune response by facilitating the migration and adhesion of neutrophils to the site of infection or injury. It is part of a protein complex on the cell surface that includes other Ly6 proteins. This group of proteins helps to modulate signal transduction for immune cell functions. Ly6G serves as an anchor through its GPI linkage aiding cell-to-cell communication and signal amplification in inflammation processes.
Ly6G is significantly integrated into the immune signaling pathways notably the toll-like receptor (TLR) signaling pathway. Through these pathways Ly6G influences neutrophil migration and activation. Closely associated proteins include CD11b and CD18 which interact with Ly6G in neutrophil functions including adhesion and transmigration. These pathways are essential for orchestrating the body's defense mechanisms against pathogens.
Ly6G plays an essential role in inflammatory conditions and autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of Ly6G can alter neutrophil behavior which contributes to disease pathology. Researchers are investigating the relationship between Ly6G and other immune-related proteins like myeloperoxidase (MPO) and elastase as these protein interactions may impact the development and progression of inflammatory diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Flow cytometric analysis of mouse blood cells cells labelling Ly6g with ab283276 at 1/50 dilution (1 μg) (Right) compared with a Rabbit monoclonal isotype control - Alexa Fluor® 488 (Left) isotype control.
Cells were stained with rabbit IgG or ab283276, then stained with anti-CD11b conjugated to BV421.
Gated on viable cells.
Flow cytometric analysis of HEK-293T (Human embryonic kidney epithelial cell) transfected with HA-tagged Ly6c overexpression construct (Left) HEK-293T transfected with HA-tagged Ly6g overexpression construct (Right) cells labelling Ly6g with ab283276 at 1/500 dilution (0.1μg) compared with a isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Ly6c (Left) and Ly6g (Right) overexpression cells were surface stained with ab283276 and anti-HA tag conjugated to Alexa Fluor®647.
ab283276 does not cross-react with Ly6c.
Gated on viable cells.
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