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AB202944

Alexa Fluor® 488 Anti-LYRIC/AEG1 antibody [EP4445]

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(1 Publication)

Rabbit Recombinant Monoclonal LYRIC/AEG1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

AEG1, LYRIC, MTDH, Protein LYRIC, 3D3/LYRIC, Astrocyte elevated gene-1 protein, Lysine-rich CEACAM1 co-isolated protein, Metadherin, Metastasis adhesion protein, AEG-1

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-LYRIC/AEG1 antibody [EP4445] (AB202944)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-LYRIC/AEG1 antibody [EP4445] (AB202944)

ab202944 staining LYRIC/AEG1 in A673 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202944 at a 1/100 dilution (shown in green) and ab202272, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in A673 cells fixed with 100% methanol (5 min).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP4445

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in A673 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LYRIC/AEG1 also known as Astrocyte Elevated Gene-1 (AEG-1) is a protein with a molecular weight of approximately 60 kDa. This protein is expressed in several tissues with notable expression in the brain liver and other organs. LYRIC/AEG1 is encoded by the MTDH gene and plays a mechanical role in regulating processes such as gene expression and cell survival. It localizes to the endoplasmic reticulum the perinuclear region and also the cell nucleus indicating a multifaceted role in cellular dynamics.
Biological function summary

LYRIC/AEG1 functions significantly in oncogenesis and tumor progression. It participates actively in gene regulation and interacts with various proteins as part of complex protein networks influencing cell survival migration and adherence. This protein is known to enhance metastasis and promote angiogenesis in malignant contexts. Researchers often study the modulation of LYRIC/AEG1 to assess its impact on cell proliferation and differentiation in cancer cells.

Pathways

LYRIC/AEG1 is associated with important signaling pathways such as the NF-κB and PI3K/Akt pathways. These pathways are critical for cell survival and proliferation and LYRIC/AEG1 modulates their activity. Through these pathways it interacts with other proteins like IκB kinase complexes and Akt coordinating cellular responses to external stimuli. LYRIC/AEG1’s influence on these pathways highlights its role in cellular adaptation to stress and potential therapeutic interventions.

LYRIC/AEG1’s role becomes apparent in its association with cancers specifically hepatocellular carcinoma and breast cancer. In these cancers LYRIC/AEG1 overexpression correlates with poor prognosis and enhanced tumor progression. Its interaction with proteins such as c-Myc and E-cadherin highlights its involvement in cancer cell metabolism and epithelial-mesenchymal transition (EMT). Understanding LYRIC/AEG1’s function in disease contexts aids in developing targeted treatment strategies for these malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Down-regulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
See full target information MTDH
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 55:1077-1089 PubMed31545408

2019

Genome profiling revealed the activation of IL2RG/JAK3/STAT5 in peripheral T‑cell lymphoma expressing the ITK‑SYK fusion gene.

Applications

Unspecified application

Species

Unspecified reactive species

Lei-Lei Zhang,Hua-Xiong Pan,Yi-Xuan Wang,Tao Guo,Lin Liu
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Product promise

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