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AB205812

Alexa Fluor® 488 Anti-M6PR (cation independent) antibody [2G11] - Lysosome Membrane Marker

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(1 Publication)

Mouse Monoclonal M6PR (cation dependent) antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Cation-dependent mannose-6-phosphate receptor, CD Man-6-P receptor, CD-MPR, 46 kDa mannose 6-phosphate receptor, MPR 46

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-M6PR (cation independent) antibody [2G11] - Lysosome Membrane Marker (AB205812)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-M6PR (cation independent) antibody [2G11] - Lysosome Membrane Marker (AB205812)

ab205812 staining M6PR in wild-type HAP1 cells (top panel) and IGF2R knockout HAP1 cells (bottom panel). The cells were fixed with 4% PFA for 10 min., permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab205812 at 5μg/ml and ab195889 (Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 594)) at 1/250 dilution (shown in pseudocolour red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-M6PR (cation independent) antibody [2G11] - Lysosome Membrane Marker (AB205812)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-M6PR (cation independent) antibody [2G11] - Lysosome Membrane Marker (AB205812)

ab205812 staining M6PR (cation independent) in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205812 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

  • Unconjugated

    Anti-M6PR (cation independent) antibody [2G11]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-M6PR (cation independent) antibody [2G11] - Lysosome Membrane Marker

  • 519 FITC

    FITC Anti-MHC Class II antibody [2G11]

  • 578 PE

    PE Anti-MHC Class II antibody [2G11]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2G11

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

This antibody is shown to recognize an epitope in the extracellular domain of Mannose 6 Phosphate Receptor.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Primates": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The cation-independent mannose 6-phosphate receptor (M6PR) is an important player in cellular transport mechanisms. Also known as the insulin-like growth factor 2 receptor (IGF2R) this protein weighs approximately 300 kDa. M6PR is present on the membrane of lysosomes and works as a lysosome membrane marker. It functions as a sorting receptor in the Golgi apparatus and endosomes facilitating the transport of lysosomal enzymes from the Golgi to lysosomes and back for function and degradation.
Biological function summary

The M6PR enables essential cellular processes by mediating the trafficking of enzymes important to lysosomal function. This receptor takes part in recognizing and binding proteins tagged with mannose 6-phosphate signals allowing their transport to lysosomes. IGF2R engages in forming transient complexes with these enzymes within the cellular trafficking circuitry therefore ensuring the proper delivery of enzyme cargo to its target destination.

Pathways

M6PR fits importantly within lysosomal enzyme transport and the insulin-like growth factor (IGF) pathway. It partially regulates the homeostasis of IGF2 by facilitating its degradation modulating cell proliferation growth and development. Steric interactions with IGF2 and other proteins like mannose receptor homologs and IGF-binding proteins outline its influence within these pathways highlighting its multifunctionality.

M6PR connects to certain cancers and lysosomal storage disorders. Dysfunctional pathway interactions or mutations within IGF2R often relate with types of cancer such as breast and liver cancers due to its influence on IGF2 regulation and cell proliferation. Additionally it associates with lysosomal storage diseases as lysosome-related pathologies emerge from impaired enzyme transport reflecting the M6PR's intricate role in cellular homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.
See full target information M6PR
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:8006 PubMed31142756

2019

HIV Infection Induces Extracellular Cathepsin B Uptake and Damage to Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Yisel M Cantres-Rosario,Sarah C Ortiz-Rodríguez,Aemil G Santos-Figueroa,Marines Plaud,Karla Negron,Bianca Cotto,Dianne Langford,Loyda M Melendez
View all publications
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Product promise

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