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AB270905

Alexa Fluor® 488 Anti-MAGEA1 antibody [SP188]

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Rabbit Monoclonal MAGEA1 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

MAGE1, MAGE1A, MAGEA1, Melanoma-associated antigen 1, Antigen MZ2-E, Cancer/testis antigen 1.1, MAGE-1 antigen, CT1.1

1 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-MAGEA1 antibody [SP188] (AB270905)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-MAGEA1 antibody [SP188] (AB270905)

Flow cytometry overlay histogram showing left A431 positive cells and right negative MCF7 cells stained with ab270905 (red line). The cells were fixed with 80 % methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab270905) (1x 106 in 100μL at 1μg/mL (1/500)) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor 488 ® (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP188

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAGEA1 also known as MAGE-1 is a member of the MAGE (Melanoma Antigen Gene) family of proteins. It has a molecular weight of approximately 39 kDa. MAGEA1 is expressed mainly in male germline tissues and in various cancer cells but not in adult somatic tissues. The protein functions mechanically by interacting with specific molecules in cells although detailed mechanisms are still under investigation.
Biological function summary

The protein encoded by MAGEA1 participates in cellular processes linked to cancer development and progression. It does not function as part of a larger complex but interacts directly with certain cellular pathways. In particular the expression of MAGEA1 is often associated with transformation events and uncontrolled cell proliferation underlining its role in oncogenesis.

Pathways

MAGEA1 plays roles in oncogenic and apoptotic pathways. It can influence the p53 signaling pathway important for cell cycle regulation and apoptosis. The interaction with other proteins such as p53 and potentially with the NF-kB pathway highlights how MAGEA1 might modulate the apoptotic response. MAGEA1 impacts cellular mechanisms by affecting apoptosis and proliferation through these pathways.

MAGEA1 is strongly related to cancer particularly melanoma and some other tumor types. Its expression in cancers makes MAGEA1 a target for cancer immunotherapy. MAGEA1 associates with proteins like p53 in certain cancers and can alter the tumor suppressive characteristics. Its role in these diseases presents MAGEA1 as a biomarker for cancer diagnosis and treatment adding clinical significance to its study.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
See full target information MAGEA1
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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