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AB216360

Alexa Fluor® 488 Anti-MALT1/MLT antibody [EP603Y]

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(1 Publication)

Rabbit Recombinant Monoclonal MALT1/MLT antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MLT, MALT1, Mucosa-associated lymphoid tissue lymphoma translocation protein 1, MALT lymphoma-associated translocation, Paracaspase

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MALT1/MLT antibody [EP603Y] (AB216360)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MALT1/MLT antibody [EP603Y] (AB216360)

ab216360 staining MALT1/MLT in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab216360 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

  • Unconjugated

    Anti-MALT1/MLT antibody [EP603Y]

  • Carrier free

    Anti-MALT1/MLT antibody [EP603Y] - BSA and Azide free

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-MALT1 antibody [EP603Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-MALT1 antibody [EP603Y]

  • 660 APC

    APC Anti-MALT1/MLT antibody [EP603Y]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-MALT1/MLT antibody [EP603Y]

  • 578 PE

    PE Anti-MALT1/MLT antibody [EP603Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-MALT1/MLT antibody [EP603Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP603Y

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protease that enhances BCL10-induced activation : acts via formation of CBM complexes that channel adaptive and innate immune signaling downstream of CARD domain-containing proteins (CARD9, CARD11 and CARD14) to activate NF-kappa-B and MAP kinase p38 pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (PubMed : 11262391, PubMed : 18264101, PubMed : 24074955). Mediates BCL10 cleavage : MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion (PubMed : 11262391, PubMed : 18264101). Involved in the induction of T helper 17 cells (Th17) differentiation (PubMed : 11262391, PubMed : 18264101). Cleaves RC3H1 and ZC3H12A in response to T-cell receptor (TCR) stimulation which releases their cooperatively repressed targets to promote Th17 cell differentiation (By similarity). Also mediates cleavage of N4BP1 in T-cells following TCR-mediated activation, leading to N4BP1 inactivation (PubMed : 31133753). May also have ubiquitin ligase activity : binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity (PubMed : 14695475).
See full target information MALT1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 59:7006-7024 PubMed36070120

2022

Inhibition of cGAS in Paraventricular Nucleus Attenuates Hypertensive Heart Injury Via Regulating Microglial Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Chengzhi Han,Xinyi Qian,Xiaorong Ren,Shutian Zhang,Li Hu,Jingyao Li,Yijun Huang,Renhui Huang,Kokwin Ooi,Hong Lin,Chunmei Xia
View all publications
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Sample Prep & Detection Kits

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Sample Prep & Detection Kits

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Sample Prep & Detection Kits

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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