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AB313398

Alexa Fluor® 488 Anti-CD206 antibody [EPR22489-7]

4

(1 Review)

|

(5 Publications)

Rabbit Recombinant Monoclonal Mannose Receptor antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications.

View Alternative Names

CD206, CLEC13D, CLEC13DL, MRC1L1, MRC1, Macrophage mannose receptor 1, MMR, C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like, Human mannose receptor, Macrophage mannose receptor 1-like protein 1, hMR

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD206 antibody [EPR22489-7] (AB313398)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD206 antibody [EPR22489-7] (AB313398)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling CD206 with ab313398 at 1/50 (10.0 ug/ml). Negative control : no staining on human skeletal muscle. The section was incubated with ab313398 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD206 antibody [EPR22489-7] (AB313398)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD206 antibody [EPR22489-7] (AB313398)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling CD206 with ab313398 at 1/50 (10.0 ug/ml). Membranous staining on immune cells in human colon. The section was incubated with ab313398 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD206 antibody [EPR22489-7] (AB313398)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD206 antibody [EPR22489-7] (AB313398)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling CD206 with ab313398 at 1/50 (10.0 ug/ml). Membranous staining on stromal cells in human liver. The section was incubated with ab313398 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22489-7

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CD206 or MRC1 is a type of C-type lectin receptor with a molecular weight of 175 kDa. It is expressed on the surface of macrophages and dendritic cells. This receptor binds to complex carbohydrates like mannose and fucose found on pathogen surfaces playing an important role in their recognition. CD206 acts as endocytic receptors aiding in the internalization and degradation of pathogens.
Biological function summary

The CD206 functions in pathogen recognition and clearance by binding to specific carbohydrates on the pathogen's surface. It is not part of a larger molecular complex but works independently to facilitate phagocytosis. The receptor is essential in immune responses allowing macrophages and dendritic cells to efficiently capture and process antigens for presentation to T cells. This enables the initiation of adaptive immune responses.

Pathways

Researchers know that the CD206 plays an important role in immune signaling pathways particularly in antigen processing and presentation. It interacts with proteins involved in these pathways including those necessary for the internalization and degradation of bound antigens. Moreover it works alongside scavenger receptors contributing to the regulation of immune and inflammatory responses.

The CD206 is often linked to pulmonary tuberculosis and certain types of cancer. When macrophages express this receptor it can modulate immune responses against Mycobacterium tuberculosis by aiding in its recognition. In cancer altered expression of CD206 can indicate tumor-associated macrophages which promote tumor growth by suppressing immune responses. These connections highlight the receptor's potential as a diagnostic marker and therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains.. (Microbial infection) Acts as a phagocytic receptor for bacteria, fungi and other pathogens.. (Microbial infection) Acts as a receptor for Dengue virus envelope protein E.. (Microbial infection) Interacts with Hepatitis B virus envelope protein.
See full target information MRC1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:776 PubMed40640851

2025

Knocking down hypoxia-induced Semaphorin 6B may attenuate the progression of cervical cancer through regulating macrophage M2 polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyan Yi,Sha Hu,Wenhan Li,Jing Cai,Liqiong Cai,Lufang Wang

Scientific reports 15:14786 PubMed40295726

2025

Epicardial adipose tissue features as a biomarker and therapeutic target in coronary artery disease.

Applications

Unspecified application

Species

Unspecified reactive species

Amir Ghaffari Jolfayi,Ahmad Tashakori Beheshti,Seyyed Mohammadjavad Hosseini,Amir Akbar Fakhrabadi,Bahram Mohebbi,Mahshid Malakootian,Majid Maleki,Hamidreza Pouraliakbar,Saeid Hosseini,Maedeh Arabian

European journal of medical research 30:144 PubMed40022250

2025

The mechanism of high mobility group box-1 in the proliferation and macrophage polarization in esophageal squamous cell carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liling Luo,Chao Jiang,Songxi Xie

PeerJ 13:e18926 PubMed39902328

2025

Fructose 1,6-bisphosphatase 1 is a potential biomarker affecting the malignant phenotype and aerobic glycolysis in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Weihong Lu,Guozheng Huang,Yihan Yu,Xia Zhai,Xiangfeng Zhou

Biomedicines 12: PubMed38927412

2024

M2 Macrophages Guide Periosteal Stromal Cell Recruitment and Initiate Bone Injury Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Dazhuang Lu,Yingfei Zhang,Shimin Liang,Yang Li,Jia Qing,Lanxin Gu,Xiuyun Xu,Zeying Wang,Xin Gao,Hao Liu,Xiao Zhang,Yongsheng Zhou,Ping Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com