JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB208563

Alexa Fluor® 488 Anti-mH2A1 antibody [EPR9359(2)]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal mH2A1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

H2AFY, MACROH2A1, Core histone macro-H2A.1, Histone macroH2A1, mH2A1, Histone H2A.y, Medulloblastoma antigen MU-MB-50.205, H2A/y

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-mH2A1 antibody [EPR9359(2)] (AB208563)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-mH2A1 antibody [EPR9359(2)] (AB208563)

ab208563 staining mH2A1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208563 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 100% methanol (5 min)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-mH2A1 antibody [EPR9359(2)] (AB208563)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-mH2A1 antibody [EPR9359(2)] (AB208563)

ab208563 staining mH2A1 in wild-type HAP1 cells (top panel) and H2AFY knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab208563 at 1/500 dilution (shown in green) and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9359(2)

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p>This product gave a positive signal in cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MH2A1 also known as macroH2A1 is a histone variant with a distinguished structure characterized by an extended C-terminal macro domain. It has a molecular mass of approximately 42-45 kDa. This histone variant is widely expressed across various tissues showing particularly high levels in differentiated cells. mH2A1 incorporates into chromatin impacting nucleosome stability and chromatin dynamics. Researchers have also identified an isoform known as H2AFY which further highlights the structural complexity and versatile roles of mH2A1 in cellular regulation.
Biological function summary

MH2A1 plays significant roles in chromatin remodeling and gene expression regulation. It is part of the nucleosome replacing canonical histone H2A and influencing the accessibility of chromatin to transcriptional machinery. Through this function mH2A1 can repress or activate gene expression depending on cellular context and interacting partners. Its incorporation into chromatin is associated with the silencing of certain genomic regions such as inactive X chromosome in females with potential implications for epigenetic modifications and cellular differentiation.

Pathways

MH2A1 is notably involved in pathways regulating chromatin organization and cell differentiation. It plays a role in the Polycomb Repressive Complex 2 (PRC2) pathway affecting histone methylation and gene silencing. Furthermore mH2A1 interacts with proteins like EZH2 in PRC2 which carry out methylation at histone H3 on lysine 27. These interactions highlight mH2A1's involvement in maintaining repressive chromatin states and controlling gene expression patterns important for normal cell function.

MH2A1 is connected with cancer and metabolic diseases. Its altered expression has been observed in various cancers where it might impact tumor progression by modulating genes linked to proliferation and differentiation. mH2A1 influences pathways shared with proteins like BRAC1 that are involved in DNA repair processes. Additionally changes in mH2A1 expression are linked to metabolic disorders especially those affecting lipid metabolism and insulin responses suggesting its potential role in regulating metabolic pathways and influencing disease progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription (PubMed : 12718888, PubMed : 15621527, PubMed : 16428466). Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template (PubMed : 15897469). Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability (PubMed : 15897469). DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation (PubMed : 15897469). Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes (PubMed : 12718888, PubMed : 16428466). Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin (PubMed : 16107708, PubMed : 16428466).. Isoform 1. Isoform that specifically binds poly-ADP-ribose and O-acetyl-ADP-ribose and plays a key role in NAD(+) metabolism (PubMed : 15902274). Able to bind to the ends of poly-ADP-ribose chains created by PARP1 and cap them (By similarity). This prevents PARP1 from further addition of ADP-ribose and thus limits the consumption of nuclear NAD(+), allowing the cell to maintain proper NAD(+) levels in both the nucleus and the mitochondria to promote proper mitochondrial respiration (By similarity). Increases the expression of genes involved in redox metabolism, including SOD3 (PubMed : 23022728).. Isoform 2. In contrast to isoform 1, does not bind poly-ADP-ribose (PubMed : 15902274). Represses SOD3 gene expression (PubMed : 23022728).
See full target information MACROH2A1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neurobiology of disease 146:105130 PubMed33065281

2020

TDP-43 and Tau Oligomers in Alzheimer's Disease, Amyotrophic Lateral Sclerosis, and Frontotemporal Dementia.

Applications

Unspecified application

Species

Unspecified reactive species

Mauro Montalbano,Salome McAllen,Filippa Lo Cascio,Urmi Sengupta,Stephanie Garcia,Nemil Bhatt,Anna Ellsworth,Eric A Heidelman,Omar D Johnson,Samantha Doskocil,Rakez Kayed
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB37264

Anti-mH2A1 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody (AB37264)

  • 4
  • 5
Primary Antibodies

AB137117

Anti-mH2A1 antibody [EPR9358]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody [EPR9358] (AB137117)

  • 4
  • 1
Primary Antibodies

AB91528

Anti-mH2A1 antibody [14G7]

KO Validated

Flow Cytometry (Intracellular) - Anti-mH2A1 antibody [14G7] (AB91528)

  • 4
  • 1

Complementary Products

Primary Antibodies

AB10158

Anti-Histone H4 antibody - ChIP Grade

BOND RX™ Validated

Western blot - Anti-Histone H4 antibody - ChIP Grade (AB10158)

  • 4
  • 21
Primary Antibodies

AB18255

Anti-Histone H2A antibody - ChIP Grade

Western blot - Anti-Histone H2A antibody - ChIP Grade (AB18255)

  • 5
  • 23
Primary Antibodies

AB1790

Anti-Histone H2B antibody - ChIP Grade

BOND RX™ Validated

Western blot - Anti-Histone H2B antibody - ChIP Grade (AB1790)

  • 5
  • 28
Secondary Antibodies

AB150175

Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed (AB150175)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com