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AB307620

Alexa Fluor® 488 Anti-MIP-1 alpha/CCL3 + CCL3L1 antibody [EPR22529-19]

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(1 Publication)

Rabbit Recombinant Monoclonal MIP-1 alpha/CCL3 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

G0S19-1, MIP1A, SCYA3, CCL3, C-C motif chemokine 3, G0/G1 switch regulatory protein 19-1, Macrophage inflammatory protein 1-alpha, PAT 464.1, SIS-beta, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein, MIP-1-alpha

2 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-MIP-1 alpha/CCL3 + CCL3L1 antibody [EPR22529-19] (AB307620)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-MIP-1 alpha/CCL3 + CCL3L1 antibody [EPR22529-19] (AB307620)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (human monocytic leukemia monocyte) treated with 100ng/ml PMA for 56 hours, add 1 μg/ml LPS for another 16 hours (Red) / Untreated control (Green) cells labelling MIP-1 alpha/CCL3 + CCL3L1 with ab307620 at 1/50 dilution (1μg) (Red) and Green (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MIP-1 alpha/CCL3 + CCL3L1 antibody [EPR22529-19] (AB307620)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MIP-1 alpha/CCL3 + CCL3L1 antibody [EPR22529-19] (AB307620)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling MIP-1 alpha/CCL3 + CCL3L1 with ab307620 at 1/250 (2.0 μg/ml) dilution. Confocal image showing cytoplasmic staining in THP-1 treated with Phorbol-12-myristate-13-acetate (100ng/ml) for 56 h, followed by adding Lipopolysaccharide (1μg/ml) for a further 16 h. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

  • Carrier free

    Anti-MIP-1 alpha/CCL3 + CCL3L1 antibody [EPR22529-19] - BSA and Azide free

  • Unconjugated

    Anti-MIP-1 alpha/CCL3 + CCL3L1 antibody [EPR22529-19]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-MIP-1 alpha/CCL3 + CCL3L1 antibody [EPR22529-19]

  • 578 PE

    PE Anti-MIP-1 alpha/CCL3 + CCL3L1 antibody [EPR22529-19]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22529-19

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Human, Mouse

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB229400

Human MIP-1 alpha/CCL3 ELISA Kit, Fluorescent

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Sandwich ELISA - Human MIP-1 alpha/CCL3 ELISA Kit, Fluorescent (AB229400)

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Primary Antibodies

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Anti-CCL4/MIP-1 beta antibody [EP521Y]

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  • 1
  • 1
Primary Antibodies

AB259372

Anti-CCL3 / MIP-1 alpha + CCL3L1 antibody [EPR23751-54]

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  • 0
  • 0
Primary Antibodies

AB303458

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Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-IL-6 antibody [EPR21711] (AB303458)

  • 0
  • 0
Primary Antibodies

AB183218

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Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [EPR19147] (AB183218)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIP-1 alpha also known as CCL3 and CCL3L1 is a chemokine with a core protein mass around 8 kDa. It is primarily expressed in activated T cells monocytes and lymphocytes. CCL3 plays a role in chemotaxis by binding to receptors CCR1 and CCR5 on the surface of target cells. This interaction leads to cell signaling events that control the migration and activation of specific immune cells to sites of inflammation or infection.
Biological function summary

The molecule acts as an important mediator in the immune response by recruiting and activating neutrophils eosinophils and other leukocytes. It supports inflammatory responses particularly in allergic reactions and autoimmunity. While not a part of a traditional complex MIP-1 alpha directly interacts with its receptors to initiate immune signaling pathways. Its expression can be upregulated by pro-inflammatory cytokines thereby enhancing its biological effects during immune system activation.

Pathways

MIP-1 alpha is closely intertwined in the chemokine signaling pathway and plays an important role in the inflammatory response pathway. Within these pathways MIP-1 alpha coordinates with proteins like CCL5 (RANTES) and interleukin-8 facilitating the recruitment of immune cells. Its function provides a pivotal control point in the migration and activation of these cells influencing the overall immune response.

MIP-1 alpha is strongly associated with rheumatoid arthritis and human immunodeficiency virus (HIV) infection. The chemokine contributes to the inflammatory milieu present in rheumatoid arthritis by promoting the recruitment of inflammatory cells to the synovial membrane. In HIV MIP-1 alpha competes with the virus for binding to CCR5 receptors influencing disease progression. It shares its target receptor CCR5 with proteins such as CCL4 (MIP-1 beta) highlighting its importance in the context of viral diseases and autoimmune conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
See full target information CCL3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1354926 PubMed39372399

2024

Altered immune cell in human severe acute pancreatitis revealed by single-cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Zheyi Wu,Shijie Wang,Zhiheng Wu,Junjie Tao,Lei Li,Chuanming Zheng,Zhipeng Xu,Zhaohui Du,Chengpu Zhao,Pengzhen Liang,Aman Xu,Zhenjie Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com