Rabbit Recombinant Monoclonal MNDA antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
IHC-P | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|
Human | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
May act as a transcriptional activator/repressor in the myeloid lineage. Plays a role in the granulocyte/monocyte cell-specific response to interferon. Stimulates the DNA binding of the transcriptional repressor protein YY1.
Myeloid cell nuclear differentiation antigen, MNDA
Rabbit Recombinant Monoclonal MNDA antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
MNDA known as Myeloid Cell Nuclear Differentiation Antigen has a mass of approximately 45 kDa. Expression occurs mainly in myeloid cells including monocytes and granulocytes. It is encoded by the MNDA gene situated on chromosome 1q22. MNDA belongs to the HIN-200 family of proteins which are involved in various cellular activities. The protein contains a DNA-binding domain suggesting its role in transcriptional regulation.
The function of MNDA is critical for the immune response and cellular differentiation. MNDA participates in regulating gene transcription in response to external stimuli. It operates in complex with other nuclear proteins to modulate the immune and inflammatory response aiming for a precise cellular behavior. MNDA's presence in myeloid cells signifies its role in the innate immune system contributing significantly to cell maturation and defense mechanisms.
MNDA influences gene expression modifications tied to key immune response pathways. It has a noted involvement in the Toll-like receptor (TLR) signaling pathway where it helps mediate responses to pathogens. MNDA interacts with proteins such as TLR4 which plays a vital role in innate immunity. Furthermore MNDA participates in the interferon regulatory factor (IRF) pathway impacting type I interferon production and immune regulation.
MNDA has associations with myeloid malignancies and autoimmune conditions. Abnormal expression or mutation of MNDA might contribute to disease pathology in acute myeloid leukemia (AML) where it may function alongside proteins like RUNX1 a transcription factor important in hematopoiesis. Additionally MNDA expressions and mutations link to systemic lupus erythematosus (SLE) where its regulatory impact on the immune response may be disrupted influencing inflammatory pathways and immune system regulation.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
MNDA Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human skeletal muscle using rabbit Anti-MNDA antibody
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling MNDA with ab324034 at 1/100 (5.0 ug/ml) dilution.
Negative control: no staining in human skeletal muscle.
The section was incubated with ab324034 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
MNDA Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human tonsil tissue using rabbit Anti-MNDA antibody
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MNDA with ab324034 at 1/100 (5.0 ug/ml) dilution.
Positive staining in human tonsil.
The section was incubated with ab324034 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
MNDA Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-MNDA antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1(human monocytic leukemia monocyte) cells labelling MNDA with ab324034 at 1/50 (10.0 ug/ml) dilution (Green).
Confocal image showing nuclear staining in THP-1 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Negative control: K-562 (PMID: 12112016).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).
MNDA Immunocytochemistry/ Immunofluorescence staining of Human PBMC using rabbit Anti-MNDA antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC cells labelling MNDA with ab324034 at 1/50 (10.0 ug/ml) dilution (Green).
Confocal image showing nuclear staining in subsets of human PBMCs (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).
MNDA Flow Cytometry (Intracellular) staining using rabbit Anti-MNDA antibody
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562 (human chronic myelogenous leukemia lymphoblast, Left) / THP-1 (human monocytic leukemia monocyte, Right) cells labelling MNDA with ab324034 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Negative control: K-562 (PMID: 12112016).
MNDA Flow Cytometry (Intracellular) staining of Human PBMC (human peripheral blood mononuclear cell) using rabbit Anti-MNDA antibody
Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human PBMC (human peripheral blood mononuclear cell) cells labelling MNDA with ab324034 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091) / Left isotype control.
Cells were stained with anti-CD14 conjugated to Brilliant Violet 421. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or our antibody.
MNDA Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human Hodgkin's lymphoma using rabbit Anti-MNDA antibody
Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling MNDA with ab324034 at 1/100 (5.0 ug/ml) dilution.
Positive staining in human Hodgkin's lymphoma.
The section was incubated with ab324034 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
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