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AB234075

Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control

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(1 Publication)

Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control Suitable for Flow Cyt and reacts with samples. Cited in 1 publication.

View Alternative Names

Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4

6 Images
Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (AB234075)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (AB234075)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD20 with ab316882 at 1/50 dilution (1 ug)/Right compared with a Mouse IgG1 [MOPC-21] - Isotype Control (AlexaFluor® 488) (ab234075) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Human PBMC are co-stained with CD19 conjugated Alexa Fluor®PE/Cy7 (780/60BP).
Gated on viable cell.

Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (AB234075)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (AB234075)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD11c with ab313900 at 1/500 dilution (0.1 ug)/Right compared with a Mouse IgG1 [MOPC-21] - Isotype Control (AlexaFluor® 488) (ab234075) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Cells are co-stained with CD11b conjugated to BV421. Gated on viable cells.

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (AB234075)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (AB234075)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte, Left) / Ramos (human Burkitt's lymphoma B lymphocyte, Right) cells labelling Bcl6 with ab324074 at 1/500 dilution (0.1ug) (Red) compared with a Mouse IgG1 [MOPC-21] - Isotype Control (AlexaFluor® 488) (ab234075) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Negative control : Jurkat (PMID : 8652841, 10757802).

Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (AB234075)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (AB234075)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD63 with ab318246 at 1/500 dilution (0.1ug) / Right compared with a Mouse IgG1 [MOPC-21] - Isotype Control (AlexaFluor® 488) (ab234075) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cells.
Cells are co-stained with CD14 conjugated to Alexa Fluor® 647.

Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (AB234075)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (AB234075)

Flow cytometric analysis of SK-MEL-28 (Human malignant melanoma) cells labelling CD63 with ab318246 at 1/500 dilution (0.1ug) (Red) compared with a Mouse IgG1 [MOPC-21] - Isotype Control (AlexaFluor® 488) (ab234075) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cells.

Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (AB234075)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (AB234075)

Flow cytometric analysis of human peripheral blood labeled with ab234075 at 9 μg/ml. Surface staining.

  • Unconjugated

    Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control

  • 660 APC

    APC Mouse IgG1 kappa [MOPC-21] - Isotype Control

  • 785 APC/Cy7®

    APC/Cy7® Mouse IgG1 kappa [MOPC-21] - Isotype Control

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Mouse IgG1 kappa [MOPC-21] - Isotype Control

  • Biotin

    Biotin Mouse IgG1, Kappa Monoclonal [MOPC-21] - isotype control

  • 519 FITC

    FITC Mouse IgG1 kappa [MOPC-21] - Isotype Control

  • 778 PE/Cy7®

    PE/Cy7® Mouse IgG1 kappa Isotype Control

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Mouse IgG1, Kappa Monoclonal [MOPC-21] - Isotype Control

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MOPC-21

Isotype

IgG1

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Applications

Flow Cyt

applications

Specificity

MOPC-21 immunoglobulin has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live and fixed rat and human tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with Alexa Fluor® 488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors, which upon binding to a specific antigen trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs known as antibodies mediate the effector phase of humoral immunity by blocking the interaction of infectious antigens with cellular receptors (via the antigen-binding region) and eliciting effector mechanisms that lead to pathogen neutralization (via the constant region) (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The antigen-binding region is formed by the variable domain of one heavy chain paired with the variable domain of its associated light chain. Each Ig molecule has two antigen-binding sites with remarkable affinity for a particular antigen due to V-(D)-J rearrangement, somatic hypermutations and affinity maturation of the variable domains upon antigen exposure (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region defines the Ig isotype that perform distinct sets of effector functions. B cells diversify and rearrange their Ig constant regions through class-switch recombination, a process by which the constant region is switched from one Ig isotype to another, namely from IgM and IgD to IgG, IgA and IgE (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region interacts (via the fragment crystallizable, Fc) with the Fc receptors on innate immune cells to mediate humoral effector functions. Ig gamma-4 (IgG4) isotype does not elicit antibody-dependent cellular cytotoxicity (ADDC) or complement-dependent cytotoxicity (CDC). Instead it is likely involved in immune tolerance mechanisms to allergens and parasites either by blocking IgE-antigen complex formation or by directly inhibiting mast cell degranulation through Fc receptor signaling. In the context of tumorigenesis, it may participate in immunosuppressive mechanisms.
See full target information IGHG4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 18:5407-5422 PubMed37753068

2023

Identification and Characterization of a Novel Nanobody Against Human CTGF to Reveal Its Antifibrotic Effect in an in vitro Model of Liver Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Liu,Min Zhu,Jiaojiao Chen,Junwei Gai,Jing Huang,Yingqun Zhou,Yakun Wan,Chuantao Tu
View all publications
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Product promise

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