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Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control Suitable for Flow Cyt and reacts with samples.

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Images

Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (AB234075), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (AB234075), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (AB234075), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (AB234075), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (AB234075), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Reactivity data

Application
Flow Cyt
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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4 products for Alternative Product

Target data

Function

The protein expressed by the IGHG4 gene is part of the constant region of immunoglobulin heavy chains. Immunoglobulins, or antibodies, are glycoproteins produced by B lymphocytes that can be membrane-bound or secreted. During humoral immunity's recognition phase, membrane-bound immunoglobulins act as receptors. When they bind a specific antigen, they trigger B lymphocytes to undergo clonal expansion and differentiate into plasma cells that secrete immunoglobulins. These secreted immunoglobulins then mediate the effector phase of humoral immunity to eliminate the bound antigens. Each immunoglobulin possesses two antigen binding sites, formed by the variable domains of a heavy chain and an associated light chain, allowing for high affinity binding to specific antigens. These variable domains undergo V-(D)-J rearrangement and may experience somatic hypermutations, facilitating affinity maturation after antigen exposure and selection. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4

Recommended products

Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control Suitable for Flow Cyt and reacts with samples.

Key facts

Isotype
IgG1
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Clone number
MOPC-21
Purification technique
Size-exclusion chromatography
Specificity

MOPC-21 immunoglobulin has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live and fixed rat and human tissues.

Concentration
Loading...
Purification notes

Purified antibody is conjugated with Alexa Fluor® 488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Store in the dark

Notes

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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5 product images

  • Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (ab234075), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (ab234075)

    Flow Cytometry staining using mouse Anti- antibody

    Flow cytometric analysis of human peripheral blood labeled with ab234075 at 9 μg/ml. Surface staining.

  • Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (ab234075), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (ab234075)

    Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using mouse Anti- antibody

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD11c with Alexa Fluor® 488 Anti-CD11c antibody [KB90] ab313900 at 1/500 dilution (0.1 ug)/Right compared with a Mouse IgG1 [MOPC-21] - Isotype Control (AlexaFluor® 488) (ab234075) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Cells are co-stained with CD11b conjugated to BV421. Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (ab234075), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (ab234075)

    Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using mouse Anti- antibody

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD20 with Alexa Fluor® 488 Anti-CD20 antibody [PDR78] ab316882 at 1/50 dilution (1 ug)/Right compared with a Mouse IgG1 [MOPC-21] - Isotype Control (AlexaFluor® 488) (ab234075) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Human PBMC are co-stained with CD19 conjugated Alexa Fluor®PE/Cy7 (780/60BP).

    Gated on viable cell.

  • Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (ab234075), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (ab234075)

    Flow Cytometry staining of SK-MEL-28 (Human malignant melanoma) using mouse Anti- antibody

    Flow cytometric analysis of SK-MEL-28 (Human malignant melanoma) cells labelling CD63 with Alexa Fluor® 488 Anti-CD63 antibody [KILL150A] ab318246 at 1/500 dilution (0.1ug) (Red) compared with a Mouse IgG1 [MOPC-21] - Isotype Control (AlexaFluor® 488) (ab234075) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (ab234075), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Mouse IgG1 [MOPC-21] - Isotype Control (ab234075)

    Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using mouse Anti- antibody

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD63 with Alexa Fluor® 488 Anti-CD63 antibody [KILL150A] ab318246 at 1/500 dilution (0.1ug) / Right compared with a Mouse IgG1 [MOPC-21] - Isotype Control (AlexaFluor® 488) (ab234075) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Gated on viable cells.

    Cells are co-stained with CD14 conjugated to Alexa Fluor® 647.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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