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Mouse Monoclonal MTCO1 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications.


Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (AB154477), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (AB154477), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (AB154477), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (AB154477), expandable thumbnail

Publications

Key facts

Isotype

IgG2a

Host species

Mouse

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)ICC/IF
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Predicted
Predicted
Caenorhabditis elegans
Predicted
Predicted
Cow
Predicted
Predicted
Zebrafish
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/500

Notes

ab171464 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Cow, Caenorhabditis elegans, Zebrafish

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

This product gave a positive signal in HeLa cells fixed 100% methanol (5 min)

Species

Human

Dilution info

1/1000

Notes

This product gave a positive signal in HeLa cells fixed 100% methanol (5 min)

Predicted
Predicted

Species

Rat, Cow, Caenorhabditis elegans, Zebrafish

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

4 products for Alternative Product

Target data

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Alternative names

Recommended products

Mouse Monoclonal MTCO1 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications.

Key facts

Isotype

IgG2a

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

1D6E1A8

Purification technique

Precipitation Ammonium Sulphate

Concentration
Loading...
Purification notes

Purity is near homogeneity as judged by SDS-PAGE. ab154477 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

ab154477 was previously used as a component in the MitoBiogenesis™ ICC Kit. The protocol for this kit is available here.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product was previously marketed under the MitoSciences sub-brand.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MTCO1 also known as COX1 or MT-CO1 is an important component of the mitochondrial respiratory chain’s complex IV commonly called cytochrome c oxidase. This target is encoded by mitochondrial DNA and contributes to the complex's catalytic core. It is a transmembrane protein with a noted molecular weight of approximately 57 kDa. MTCO1 is predominantly expressed in tissues with high energy demand such as cardiac and skeletal muscles due to their reliance on efficient oxidative phosphorylation.

Biological function summary

MTCO1 is vital for the final step of the electron transport chain catalyzing the transfer of electrons from cytochrome c to oxygen. This process facilitates the reduction of oxygen molecules to water. MTCO1 is an integral part of cytochrome c oxidase a multi-subunit enzyme complex important for cellular energy production. Proper function of MTCO1 supports ATP synthesis by maintaining electrochemical gradients across the mitochondrial inner membrane.

Pathways

Electrons transfer through this protein is essential for effective oxidative phosphorylation and maintaining the proton gradient necessary for ATP synthesis. MTCO1 operates in tandem with proteins like COX2 within the electron transport chain to achieve optimal energy conversion and cellular respiration. The pathway interactions of MTCO1 are critical in efficiently powering cellular activities and upholding metabolic functions throughout the body.

Associated diseases and disorders

Mutations or defects in MTCO1 have associations with various mitochondrial diseases such as Leber's Hereditary Optic Neuropathy (LHON) and mitochondrial complex IV deficiency. MTCO1 anomalies may disrupt normal function leading to impaired oxidative phosphorylation and energy deficits in cells. In LHON affected individuals can also demonstrate deficits linked to mutations in other mitochondrial genes like ND1 which further disrupt cellular energy balance and contribute to the clinical manifestations of these mitochondrial disorders.

Product promise

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4 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477)

    ab154477 staining MTCO1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 h. The cells were then incubated overnight at 4°C with ab154477 at 1/1000 dilution (shown in green) and Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor 647), at 1/250 dilution (pseudocolored in red). Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477)

    Overlay histogram showing HeLa cells stained with ab154477 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab154477, 1/100 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was mouse IgG2a Alexa Fluor® 488 (ab171464) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477)

    Immunocytochemistry with HeLa cells (100x) were stained with anti-MTCO1 Alexa-488 antibody (1.0 µg/mL, ab154477) in green and DAPI in blue, as a nuclear stain.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477)

    Immunocytochemistry with HDFn (100x) cells were stained with Anti-MTOC1 Alexa-488 antibody (1.0 µg/mL, ab154477) in green, Anti-HSP60 (1/1000, Anti-Hsp60 antibody ab46798) as red, and DAPI in blue, as a nuclear stain. Secondary antibody used was goat anti-rabbit dyelight-594 (1/1000, ab96897).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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