Alexa Fluor® 488 Anti-MTHFD2 antibody [EPR26938-20]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTHFD2 antibody [EPR26938-20] (AB317663)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MTHFD2 KO HEK293T (MTHFD2 knockout human embryonic kidney epithelial cell), ab266383 cells labelling MTHFD2 with ab317663 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in wildtype HEK293T cells and no staining in MTHFD2 knockout HEK293T (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-MTHFD2 antibody [EPR26938-20] (AB317663)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MTHFD2 with ab317663 at 1/100 (5.0 ug/ml) dilution.

Positive staining on human tonsil.The section was incubated with ab317663 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-MTHFD2 antibody [EPR26938-20] (AB317663)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling MTHFD2 with ab317663 at 1/100 (5.0 ug/ml) dilution.

Positive staining on human breast cancer.The section was incubated with ab317663 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-MTHFD2 antibody [EPR26938-20] (AB317663)

Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling MTHFD2 with ab317663 at 1/100 (5.0 ug/ml) dilution.

Positive staining on mouse pancreas. The section was incubated with ab317663 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTHFD2 antibody [EPR26938-20] (AB317663)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MEF (mouse embryo fibroblast) cells labelling MTHFD2 with ab317663 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in MEF cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).