AB199781

Alexa Fluor® 488 Anti-Musashi 1 / Msi1 antibody [EP1302]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal Musashi 1 / Msi1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

RNA-binding protein Musashi homolog 1, Musashi-1, MSI1

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Musashi 1 / Msi1 antibody [EP1302] (AB199781)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Musashi 1 / Msi1 antibody [EP1302] (AB199781)

ab199781 staining Musashi 1 / Msi1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199781 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

578 PE

PE Anti-Musashi 1 / Msi1 antibody [EP1302]
660 APC

APC Anti-Musashi 1 / Msi1 antibody [EP1302]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Musashi 1 / Msi1 antibody [EP1302]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Musashi 1 / Msi1 antibody [EP1302]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Musashi 1 / Msi1 antibody [EP1302]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Musashi 1 / Msi1 antibody [EP1302]
Unconjugated

Anti-Musashi 1 / Msi1 antibody [EP1302]
Carrier free

Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1302

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min).</p>" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Quail": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Musashi 1 also known as Msi1 is an RNA-binding protein with a mass of about 39 kDa. Msi1 is present in high levels in neural tissue and certain stem cells. It works mechanically by recognizing and binding to specific RNA sequences impacting the fate of the mRNA. Musashi 1 regulates the translation and stability of its target mRNA affecting protein synthesis at the post-transcriptional level. Its expression is not limited to neural tissues but is also detected in other tissues where stem or progenitor cells are present.

Biological function summary

In neural stem cells and other tissue types Musashi 1 plays an important role in maintaining stem cell identity. Musashi 1 forms part of a complex that controls the translation of mRNAs involved in cell fate decisions. It acts by repressing or activating the translation of key regulatory proteins that guide stem cell maintenance and differentiation processes. This protein is also involved in cellular proliferation by influencing the translation of mRNAs tied to cell cycle regulation.

Pathways

The function of Musashi 1 lies within critical signaling pathways such as Notch and Wnt. It facilitates these pathways by regulating the translation of downstream effectors which are necessary for cellular communication and differentiation. Musashi 1 associates with proteins like Numb a known Notch signaling inhibitor and β-catenin from the Wnt pathway modulating their effects on cell cycle progression and stem cell fate.

Musashi 1’s abnormal expression is often linked to conditions like glioblastoma and colorectal cancer. High levels of Musashi 1 can promote tumor growth due to its role in cell proliferation and differentiation pathways. In glioblastoma its expression relates closely with other oncogenic proteins such as Numb and Notch altering normal regulatory mechanisms. In colorectal cancer Musashi 1’s interaction with β-catenin further drives tumorigenesis highlighting its potential as a target for therapeutic interventions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).

See full target information MSI1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy oncolytics 20:147-165 PubMed33575478

2021

Anti-oncogenic activities exhibited by paracrine factors of MSCs can be mediated by modulation of and genes in glioma SCs .

Applications

Unspecified application

Species

Unspecified reactive species

Nazneen Aslam,Elham Abusharieh,Duaa Abuarqoub,Dema Ali,Dana Al-Hattab,Suha Wehaibi,Ban Al-Kurdi,Fatima Jamali,Walhan Alshaer,Hanan Jafar,Abdalla S Awidi

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-Musashi 1 / Msi1 antibody [EP1302]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Musashi 1 / Msi1 antibody [EP1302] (AB199781)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Product promise