Alexa Fluor® 488 Anti-Myc tag antibody [9E10]

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (AB202008)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labelling HLA DMB with primary antibody anti-HLA DMB (ab131273) at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution. Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged HLA-DMB expression vector, no staining in 293T cells transfected with myc-tagged NEK6 expression vector. Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) (ab202008) was used as counterstain at 1/100 dilution. The nuclear counter stain is DAPI (blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (AB202008)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Uteroglobin with ab322394 at 1/250 (2.0 ug/ml) dilution.

Confocal image showing positive staining in 293T cells transfected with a human Uteroglobin expression vector containing a myc-his tag (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (AB202008)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with myc tagged EMR1/ADGRE1 construct cells labelling EMR1/ADGRE1 with ab322230 at 1/100 (5.0 ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cell line transfected with a myc-tagged EMR1/ADGRE1 expression vector (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (AB202008)

Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-tagged TMEM119 expression vector labelling Myc-tag with ab202008 at 1/500 dilution (right) compared with Mouse IgG1 (monoclonal) Alexa Fluor^® 488 (left). Cells were surface-stained with TMEM119 antibody (ab210405), then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor^® 488 conjugated Myc-tag antibody (ab202008) and Alexa Fluor^® 647 conjugated anti-Rabbit secondary antibody (for TMEM119). Only TMEM119 (+) population showed Myc-tag positive staining.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (AB202008)

ab202008 staining Myc-tag (shown in green) in 293T cells transfected with TMEM119 with myc tag. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202008 at 1/100 dilution and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue) and Alexa Fluor^® 647 conjugated control Myc-tag antibody (different clone) is pseudocolored in white.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).