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AB314162

Alexa Fluor® 488 Anti-Myelin Basic Protein antibody [EPR21188]

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Rabbit Recombinant Monoclonal Myelin Basic Protein antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-Fr and reacts with Mouse, Rat samples.

View Alternative Names

Myelin basic protein, MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein

2 Images
Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Myelin Basic Protein antibody [EPR21188] (AB314162)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Myelin Basic Protein antibody [EPR21188] (AB314162)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling Myelin Basic Protein with ab314162 at 1/50 (10.0 ug/ml) dilution (Green). Confocal image showing positive staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab314162 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Myelin Basic Protein antibody [EPR21188] (AB314162)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Myelin Basic Protein antibody [EPR21188] (AB314162)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Myelin Basic Protein with ab314162 at 1/50 (10.0 ug/ml) dilution (Green). Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab314162 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Myelin Basic Protein antibody [EPR21188]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Myelin Basic Protein antibody [EPR21188]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Myelin Basic Protein antibody [EPR21188]

  • Unconjugated

    Anti-Myelin Basic Protein antibody [EPR21188] - Oligodendrocyte Marker

  • Carrier free

    Anti-Myelin Basic Protein antibody [EPR21188] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21188

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The myelin basic protein also referred to as MBP or MBP protein plays an important mechanical role in the central nervous system. It is a major component in the formation and stability of the myelin sheath which wraps around nerve fibers to ensure efficient neural signaling. The molecular weight of MBP can vary due to alternative splicing; one common form has a mass of about 18.5 kDa. MBP is expressed predominantly in oligodendrocytes within the brain and spinal cord where it helps form the myelin sheath. MBP2 is another variant that may be mentioned in some contexts.
Biological function summary

MBP is not just a passive structural element; it binds and interacts with lipids and other proteins to maintain the compact structure of the myelin sheath. MBP forms part of a complex that includes various other proteins and lipids ensuring the stability and function of myelin. Misfolding or altered expression of MBP can compromise myelin integrity affecting the insulation of neural pathways and signal transmission.

Pathways

MBP is an essential component in the pathways of myelination and neural plasticity. It plays a role in the signaling pathways that stimulate the development and maintenance of the myelin sheath. The protein interacts with other elements of the myelin sheath such as proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) to coordinate its formation and repair processes.

MBP is linked closely to multiple sclerosis (MS) and other demyelinating conditions. Damage to or autoimmunity against MBP leads to the degradation of the myelin sheath contributing to the neurological symptoms in MS. The protein interacts with elements of the immune system with autoantibodies targeting MBP often found in MS patients. Another disorder associated with MBP dysfunction is Charcot-Marie-Tooth disease where abnormal MBP expression and structure can impact nerve function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
See full target information MBP
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com