Alexa Fluor® 488 Anti-Myosin heavy chain antibody [A4.1025]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Myosin heavy chain antibody [A4.1025] (AB315962)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Myosin (MYH 1/2) with ab315962 at 1/100 (5.0 ug/ml) dilution.

Cytoplasmic staining on human skeletal muscle. The section was incubated with ab315962 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Myosin heavy chain antibody [A4.1025] (AB315962)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Myosin (MYH 1/2) with ab315962 at 1/100 (5.0 ug/ml) dilution.

Cytoplasmic staining on human cardiac muscle. The section was incubated with ab315962 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Myosin heavy chain antibody [A4.1025] (AB315962)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Myosin (MYH 1/2) with ab315962 at 1/100 (5.0 ug/ml) dilution.

Cytoplasmic staining on mouse skeletal muscle. The section was incubated with ab315962 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

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Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Myosin heavy chain antibody [A4.1025] (AB315962)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh) tissue labeling Myosin (MYH 1/2) with ab315962 at 1/500 (1.0 ug/ml) dilution (Green).

Confocal image showing positive staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab315962 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Myosin heavy chain antibody [A4.1025] (AB315962)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (fresh) tissue labeling Myosin (MYH 1/2) with ab315962 at 1/500 (1.0 ug/ml) dilution (Green).

Confocal image showing positive staining on rat skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab315962 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Myosin heavy chain antibody [A4.1025] (AB315962)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Myosin (MYH 1/2) with ab315962 at 1/100 (5.0 ug/ml) dilution.

Cytoplasmic staining on rat skeletal muscle. The section was incubated with ab315962 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Myosin heavy chain antibody [A4.1025] (AB315962)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Myosin (MYH 1/2) with ab315962 at 1/500 (1.0 ug/ml) dilution (Green).

Negative control : confocal image showing no staining on rat liver (PMID : 30274168). The nuclear counterstain was DAPI (Blue). The section was incubated with ab315962 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Myosin heavy chain antibody [A4.1025] (AB315962)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Myosin (MYH 1/2) with ab315962 at 1/100 (5.0 ug/ml) dilution.

Negative control : no staining on human liver. The section was incubated with ab315962 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Myosin heavy chain antibody [A4.1025] (AB315962)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Myosin with ab315962 at 1/500 (1.0 ug/ml) dilution (Green).

Negative control : confocal image showing no staining on mouse liver (PMID : 30274168). The nuclear counterstain was DAPI (Blue). The section was incubated with ab315962 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).