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Rabbit Recombinant Monoclonal NANOG antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nanog antibody [EPR2027(2)] (AB196155), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nanog antibody [EPR2027(2)] (AB196155), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nanog antibody [EPR2027(2)] (AB196155), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Associated Products

Select an associated product type

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2 products for Alternative Version

Target data

Function

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Alternative names

Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG

Recommended products

Rabbit Recombinant Monoclonal NANOG antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2027(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Nanog also known as Nanog homeobox is a transcription factor playing an important role in maintaining the pluripotency of embryonic stem cells. The molecular weight of Nanog is approximately 35 kDa. It is found mainly in the inner cell mass of the blastocyst but it also expresses in embryonic stem cells and some adult stem cell populations. Nanog acts by binding to DNA in a sequence-specific manner to regulate gene expression essential for stem cell self-renewal and pluripotency.

Biological function summary

Nanog plays a central role in stem cell biology operating as part of a complex regulatory network. Nanog interacts with other important transcription factors like Oct4 and Sox2 forming a core pluripotency network. This network suppresses the differentiation of stem cells by regulating the expression of genes involved in cell fate decisions. By modulating the expression of different pathways Nanog ensures the maintenance of an undifferentiated state in stem cells.

Pathways

Nanog is deeply embedded in the Wnt/β-Catenin and TGF-β signaling pathways important for stem cell maintenance and differentiation. In the Wnt/β-Catenin pathway Nanog works alongside proteins like β-catenin to drive the expression of genes that promote self-renewal. Meanwhile in the TGF-β pathway Nanog acts with proteins such as Smad2/3 to balance pluripotency and differentiation signals. These pathways critically support the complex network that sustains stem cell identity and function.

Associated diseases and disorders

Nanog's expression relates closely to its role in various cancers such as glioblastoma and colorectal cancer. Abnormally high levels of Nanog contribute to the tumorigenicity of cancer cells by maintaining their self-renewal and undifferentiated state similar to its role in stem cells. Nanog interacts with proteins like p53 known for its tumor suppressor functions often leading to challenges in cancer treatment. Understanding Nanog's influence in these pathways provides valuable insights into therapeutic targets for combating cancer stem cell resilience.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nanog antibody [EPR2027(2)] (ab196155), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nanog antibody [EPR2027(2)] (ab196155)

    ab196155 staining Nanog in HeLa cells (negative). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 10% normal goat serum in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196155 at 1/100 dilution (shown in green) and Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor® 647), at 1/200 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
    Image was taken with a confocal high content analyser (Perkin Elmer, Operetta-CLS).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nanog antibody [EPR2027(2)] (ab196155), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nanog antibody [EPR2027(2)] (ab196155)

    ab196155 staining Nanog in NCCIT cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 10% normal goat serum in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196155 at 1/100 dilution (shown in green) and Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor® 647), at 1/200 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
    Image was taken with a confocal high content analyser (Perkin Elmer, Operetta-CLS).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nanog antibody [EPR2027(2)] (ab196155), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nanog antibody [EPR2027(2)] (ab196155)

    ab196155 staining Nanog in Sk-ov-3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 10% normal goat serum in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196155 at 1/50 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/200 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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