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AB197495

Alexa Fluor® 488 Anti-Nestin antibody [Rat-401]

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(4 Publications)

Mouse Monoclonal Nestin antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Rat samples. Cited in 4 publications.

View Alternative Names

Nestin, Nes

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nestin antibody [Rat-401] (AB197495)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nestin antibody [Rat-401] (AB197495)

ab197495 staining Nestin in NGF-differentiated PC-12 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab197495 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker

  • Unconjugated

    Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker

  • Carrier free

    Anti-Nestin antibody [Rat-401] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Rat-401

Isotype

IgG1

Light chain type

kappa

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Rat

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nestin also known as NES is an intermediate filament protein with a molecular weight of about 220 kDa. Nestin features prominently in cells related to the nervous system including neural stem cells and it serves as a structural component in these cells. Nestin also appears in substantial amounts during the development of the nervous system and is important for cell structural organization. It is often used as a Nestin marker in research particularly with Nestin antibodies for identifying the presence of neural stem cells and tracking their developmental course. Researchers frequently utilize techniques like Nestin IHC (immunohistochemistry) and Nestin western blot to stain and detect Nestin allowing detailed investigations into its expression in various tissues.
Biological function summary

Nestin plays a significant role in the dynamic remodeling of the cytoskeleton. It does not act as a solitary element but forms part of a larger protein complex involved in maintaining and organizing cellular structure. This property grants cells flexibility in shape and resilience during the rapid changes in development and specialization. The role of Nestin in the cellular architecture allows it to contribute to processes such as cell migration and proliferation critical during tissue development and repair.

Pathways

Nestin integrates into networks that regulate stem cell behavior and neurogenesis. It tightly interacts within the pathways involving cell cycle regulation and differentiation. For example the Wnt and Notch signaling pathways modulate its expression to influence neural stem cell fate and patterning during development. Protein interactions between Nestin and other cytoskeletal elements like vimentin facilitate its roles in these pathways adapting the cellular environment for specific developmental activities.

Nestin associates with various pathological conditions including brain tumors and muscular dystrophies. Its expression is aberrant in many forms of gliomas where it often signifies a higher grade of malignancy. Within these tumors Nestin connects to oncogenic signaling pathways involving proteins such as the epidermal growth factor receptor (EGFR). Moreover in muscular dystrophies Nestin's expression in regenerating muscle fibers illustrates its involvement in cellular repair mechanisms potentially interacting with dystrophin a protein important for muscle integrity. Research on anti-Nestin antibodies continues to clarify its role in these diseases potentially offering diagnostic or therapeutic insights.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
See full target information Nes
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of comparative neurology 530:2868-2880 PubMed35811330

2022

Uncoupling axon guidance and neuronal migration in Robo3-deficient inferior olivary neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Antonio Moreno-Bravo,Quentin Rappeneau,Sergi Roig-Puiggros,Constantino Sotelo,Alain Chédotal

The Journal of comparative neurology 527:2009-2029 PubMed30697732

2019

Shared and differential features of Robo3 expression pattern in amniotes.

Applications

Unspecified application

Species

Unspecified reactive species

François Friocourt,Peter Kozulin,Morgane Belle,Rodrigo Suárez,Nicolas Di-Poï,Linda J Richards,Paolo Giacobini,Alain Chédotal

Development (Cambridge, England) 145: PubMed29343638

2018

Commissural neurons transgress the CNS/PNS boundary in absence of ventricular zone-derived netrin 1.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Antonio Moreno-Bravo,Sergi Roig Puiggros,Heike Blockus,Chloé Dominici,Pavol Zelina,Patrick Mehlen,Alain Chédotal

Nature 545:350-354 PubMed28445456

2017

Floor-plate-derived netrin-1 is dispensable for commissural axon guidance.

Applications

Unspecified application

Species

Unspecified reactive species

Chloé Dominici,Juan Antonio Moreno-Bravo,Sergi Roig Puiggros,Quentin Rappeneau,Nicolas Rama,Pauline Vieugue,Agnes Bernet,Patrick Mehlen,Alain Chédotal
View all publications
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