Anti-Neun antibody is Alexa Fluor® 488 conjugated rabbit monoclonal antibody that is used in Neun immunocytochemistry/immunofluorescence (ICC/IF), immunohistochemistry on frozen sections (IHC-Fr), flow cytometry (intracellular), and immunohistochemistry on paraffin-embedded sections (IHC-P). Suitable for rat and human samples.
- Cited in >35 publications
- Affinity-purified using Protein A
- Use as a reliable neuronal marker
IgG
Rabbit
Alexa Fluor® 488
Ex: 495nm, Em: 519nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
ICC/IF | IHC-Fr | Flow Cyt (Intra) | IHC-P | |
---|---|---|---|---|
Human | Tested | Expected | Tested | Expected |
Mouse | Predicted | Predicted | Predicted | Predicted |
Rat | Tested | Tested | Expected | Tested |
Cat | Predicted | Predicted | Predicted | Predicted |
Common marmoset | Predicted | Predicted | Predicted | Predicted |
Dog | Predicted | Predicted | Predicted | Predicted |
Goat | Predicted | Predicted | Predicted | Predicted |
Sheep | Predicted | Predicted | Predicted | Predicted |
Zebrafish | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/50 - 1/250 | Notes - |
Species Human | Dilution info 1/50 - 1/250 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Sheep, Goat, Cat, Dog, Zebrafish, Common marmoset | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/50 | Notes Before commencing with immunostaining protocol, perform heat mediated antigen retrieval using sodium citrate buffer, pH6. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Sheep, Goat, Cat, Dog, Zebrafish, Common marmoset | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Sheep, Goat, Cat, Dog, Zebrafish, Common marmoset | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Sheep, Goat, Cat, Dog, Zebrafish, Common marmoset | Dilution info - | Notes - |
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Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).
RNA binding protein fox-1 homolog 3, Fox-1 homolog C, Neuronal nuclei antigen, NeuN antigen, RBFOX3
Anti-Neun antibody is Alexa Fluor® 488 conjugated rabbit monoclonal antibody that is used in Neun immunocytochemistry/immunofluorescence (ICC/IF), immunohistochemistry on frozen sections (IHC-Fr), flow cytometry (intracellular), and immunohistochemistry on paraffin-embedded sections (IHC-P). Suitable for rat and human samples.
- Cited in >35 publications
- Affinity-purified using Protein A
- Use as a reliable neuronal marker
IgG
Rabbit
Alexa Fluor® 488
Ex: 495nm, Em: 519nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
EPR12763
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Store in the dark
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
This supplementary information is collated from multiple sources and compiled automatically.
NeuN also known as Fox-3 or Rbfox3 serves as a vital neuronal marker and has a molecular weight of about 46-50 kDa. This nuclear protein is commonly found in neuronal cells in the central nervous system but it does not appear in glial cells. Researchers use NeuN staining as an effective tool to identify neurons in various brain regions due to its reliable expression pattern in mature neurons. The presence of NeuN can be detected using methods such as NeuN IHC (immunohistochemistry) and NeuN Western blot aiding in cellular and tissue analysis.
NeuN plays an essential role in the regulation of RNA-binding influencing the splicing and stability of transcripts. It forms part of the complex network responsible for managing neuron-specific gene expression. By interacting with other RNA-binding proteins NeuN contributes to the fine-tuning needed for proper neuronal development and function. As a result NeuN helps maintain the health and activity of nerve cells allowing them to perform complex neurological tasks.
NeuN has a significant role in neuronal differentiation and plasticity pathways. NeuN coordinates with elements of the Notch signaling pathway which is important for guiding cell fate decisions in the nervous system. It also interacts with related proteins like Fox proteins involved in splicing regulation. These pathways ensure neurons develop differentiate and function correctly within the nervous system's precise architecture.
Disruptions in NeuN expression link closely to neurodegenerative diseases such as Parkinson's disease and Amyotrophic lateral sclerosis (ALS). Abnormal NeuN expression correlates with neuron loss and dysfunction in these conditions with connectivity to other affected proteins like alpha-synuclein in Parkinson's disease. In ALS abnormal NeuN-staining patterns indicate neuronal cell death serving as a potential marker for disease progression. Studying these connections improves our understanding of disease mechanisms and aids in the development of targeted therapies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
IHC image of ab190195 staining in acetone fixed frozen tissue section of normal rat brain.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with ab190195 (1/50) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then counterstained and mounted with SlowFade® Gold Antifade Mountant with DAPI.
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 488 signal is dervied directly from bound ab190195. The separate images of ab190195 and DAPI alone, combined with the merged version of both signals, shows predominant co-localisation of the Alexa Fluor® 488 signal in the nuclei of the hippocampal granular layer.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
ab190195 staining NeuN in U87-MG cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190195 at 1/50 dilution (shown in green) and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Alexa Fluor® 594 Goat anti-Mouse secondary (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Overlay histogram showing U-87MG cells stained with ab190195 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab190195, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This antibody gave a positive signal in U-87MG fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab190195 staining NeuN in NGF-differentiated PC12 cells (7 days). The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190195 at 1/50 dilution (shown in green) and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Alexa Fluor® 594 Goat anti-Mouse secondary (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image A: composite multiplex immunohistochemistry staining using conjugates panel composed of anti-NeuN (Alexa Fluor® 488) (ab190195, 1/100 dilution, 5 µg/mL) (green), anti-GFAP (Alexa Fluor® 555) (Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] ab201735, 1/100 dilution, 5 µg/mL) (magenta) and anti-Synaptophysin (Alexa Fluor® 647) (Alexa Fluor® 647 Anti-Synaptophysin antibody [YE269] ab196166, 1/100 dilution, 5 µg/mL) (white) on Formalin/PFA-fixed paraffin-embedded sections of rat cerebellum. Nuclear DNA was labelled with DAPI (blue).
Image B: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti-NeuN (Alexa Fluor® 488) (ab190195, 1/100 dilution, 5 µg/mL).
Image C: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti-GFAP (Alexa Fluor® 555) (Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] ab201735, 1/100 dilution, 5 µg/mL).
Image D: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti-Synaptophysin (Alexa Fluor® 647) (Alexa Fluor® 647 Anti-Synaptophysin antibody [YE269] ab196166, 1/100 dilution, 5 µg/mL).
Immunohistochemistry staining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with EDTA (pH 9.0, 110°C) for 40 mins. The section was then incubated at room temperature for 1 hour and mounted using Dako Fluorescence Mounting Medium®.
Image acquisition was performed with Leica SP8 confocal microscope. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
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