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Anti-Neun antibody is Alexa Fluor® 488 conjugated rabbit monoclonal antibody that is used in Neun immunocytochemistry/immunofluorescence (ICC/IF), immunohistochemistry on frozen sections (IHC-Fr), flow cytometry (intracellular), and immunohistochemistry on paraffin-embedded sections (IHC-P). Suitable for rat and human samples.

- Cited in >35 publications
- Affinity-purified using Protein A
- Use as a reliable neuronal marker


Images

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB190195), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB190195), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB190195), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB190195), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB190195), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-FrFlow Cyt (Intra)IHC-P
Human
Tested
Expected
Tested
Expected
Mouse
Predicted
Predicted
Predicted
Predicted
Rat
Tested
Tested
Expected
Tested
Cat
Predicted
Predicted
Predicted
Predicted
Common marmoset
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Goat
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Rat

Dilution info

1/50 - 1/250

Notes

-

Species

Human

Dilution info

1/50 - 1/250

Notes

-

Predicted
Predicted

Species

Mouse, Sheep, Goat, Cat, Dog, Zebrafish, Common marmoset

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/50

Notes

Before commencing with immunostaining protocol, perform heat mediated antigen retrieval using sodium citrate buffer, pH6.

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Sheep, Goat, Cat, Dog, Zebrafish, Common marmoset

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Sheep, Goat, Cat, Dog, Zebrafish, Common marmoset

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Sheep, Goat, Cat, Dog, Zebrafish, Common marmoset

Dilution info

-

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

7 products for Alternative Version

Target data

Function

Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).

Alternative names

Recommended products

Anti-Neun antibody is Alexa Fluor® 488 conjugated rabbit monoclonal antibody that is used in Neun immunocytochemistry/immunofluorescence (ICC/IF), immunohistochemistry on frozen sections (IHC-Fr), flow cytometry (intracellular), and immunohistochemistry on paraffin-embedded sections (IHC-P). Suitable for rat and human samples.

- Cited in >35 publications
- Affinity-purified using Protein A
- Use as a reliable neuronal marker

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR12763

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NeuN also known as Fox-3 or Rbfox3 serves as a vital neuronal marker and has a molecular weight of about 46-50 kDa. This nuclear protein is commonly found in neuronal cells in the central nervous system but it does not appear in glial cells. Researchers use NeuN staining as an effective tool to identify neurons in various brain regions due to its reliable expression pattern in mature neurons. The presence of NeuN can be detected using methods such as NeuN IHC (immunohistochemistry) and NeuN Western blot aiding in cellular and tissue analysis.

Biological function summary

NeuN plays an essential role in the regulation of RNA-binding influencing the splicing and stability of transcripts. It forms part of the complex network responsible for managing neuron-specific gene expression. By interacting with other RNA-binding proteins NeuN contributes to the fine-tuning needed for proper neuronal development and function. As a result NeuN helps maintain the health and activity of nerve cells allowing them to perform complex neurological tasks.

Pathways

NeuN has a significant role in neuronal differentiation and plasticity pathways. NeuN coordinates with elements of the Notch signaling pathway which is important for guiding cell fate decisions in the nervous system. It also interacts with related proteins like Fox proteins involved in splicing regulation. These pathways ensure neurons develop differentiate and function correctly within the nervous system's precise architecture.

Associated diseases and disorders

Disruptions in NeuN expression link closely to neurodegenerative diseases such as Parkinson's disease and Amyotrophic lateral sclerosis (ALS). Abnormal NeuN expression correlates with neuron loss and dysfunction in these conditions with connectivity to other affected proteins like alpha-synuclein in Parkinson's disease. In ALS abnormal NeuN-staining patterns indicate neuronal cell death serving as a potential marker for disease progression. Studying these connections improves our understanding of disease mechanisms and aids in the development of targeted therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190195), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190195)

    IHC image of ab190195 staining in acetone fixed frozen tissue section of normal rat brain.

    Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with ab190195 (1/50) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then counterstained and mounted with SlowFade® Gold Antifade Mountant with DAPI.

    The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 488 signal is dervied directly from bound ab190195. The separate images of ab190195 and DAPI alone, combined with the merged version of both signals, shows predominant co-localisation of the Alexa Fluor® 488 signal in the nuclei of the hippocampal granular layer.

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190195), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190195)

    ab190195 staining NeuN in U87-MG cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190195 at 1/50 dilution (shown in green) and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Alexa Fluor® 594 Goat anti-Mouse secondary (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190195), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190195)

    Overlay histogram showing U-87MG cells stained with ab190195 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab190195, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This antibody gave a positive signal in U-87MG fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190195), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190195)

    ab190195 staining NeuN in NGF-differentiated PC12 cells (7 days). The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190195 at 1/50 dilution (shown in green) and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Alexa Fluor® 594 Goat anti-Mouse secondary (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190195), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190195)

    Image A: composite multiplex immunohistochemistry staining using conjugates panel composed of anti-NeuN (Alexa Fluor® 488) (ab190195, 1/100 dilution, 5 µg/mL) (green), anti-GFAP (Alexa Fluor® 555) (Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] ab201735, 1/100 dilution, 5 µg/mL) (magenta) and anti-Synaptophysin (Alexa Fluor® 647) (Alexa Fluor® 647 Anti-Synaptophysin antibody [YE269] ab196166, 1/100 dilution, 5 µg/mL) (white) on Formalin/PFA-fixed paraffin-embedded sections of rat cerebellum. Nuclear DNA was labelled with DAPI (blue).

    Image B: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti-NeuN (Alexa Fluor® 488) (ab190195, 1/100 dilution, 5 µg/mL).

    Image C: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti-GFAP (Alexa Fluor® 555) (Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] ab201735, 1/100 dilution, 5 µg/mL).

    Image D: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti-Synaptophysin (Alexa Fluor® 647) (Alexa Fluor® 647 Anti-Synaptophysin antibody [YE269] ab196166, 1/100 dilution, 5 µg/mL).

    Immunohistochemistry staining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with EDTA (pH 9.0, 110°C) for 40 mins. The section was then incubated at room temperature for 1 hour and mounted using Dako Fluorescence Mounting Medium®.

    Image acquisition was performed with Leica SP8 confocal microscope. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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