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Rabbit Recombinant Monoclonal NKR-P1C antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Mouse samples.

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Images

Flow Cytometry - Alexa Fluor® 488 Rabbit monoclonal [EPR22990-12] to NKR-P1C (AB322225), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 488 Rabbit monoclonal [EPR22990-12] to NKR-P1C (AB322225), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IF
Mouse
Tested
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Target data

Function

Plays a stimulatory role on natural killer (NK) cells cytotoxicity. Activation by cross-linking of the receptor induces Ca(2+) mobilization and interferon-gamma production.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal NKR-P1C antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Mouse samples.

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22990-12

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NKR-P1C also known as NK1.1 is a C-type lectin-like receptor expressed on the surface of natural killer (NK) cells and some T cells. In mice this protein has a molecular mass of approximately 30 to 46 kDa. Researchers often focus on its distribution in cellular compartments which includes lymphoid tissues such as the spleen and thymus. This receptor is known for playing a role in the regulation of immune responses by enabling NK cells to recognize and eliminate target cells.

Biological function summary

NKR-P1C is critical in the activation and inhibition of natural killer cell functions. This protein does not operate alone but often functions as part of an immune receptor complex. It contributes to the balance between activating and inhibitory signals that determine NK cell responses. NKR-P1C can bind to ligands that influence the cytotoxic activity of these cells toward infected or malignant cells.

Pathways

NKR-P1C integrates into important immune signaling pathways such as the NK cell-mediated cytotoxicity pathway. This pathway involves interactions with other receptors like Ly49 and NKG2D which modulate NK cell activities. NKR-P1C plays an essential role in transmitting signals that lead to the release of cytokines and the destruction of afflicted cells coordinating with these receptors to execute immune functions.

Associated diseases and disorders

NKR-P1C has associations with cancer and viral infections where it influences NK cell interventions against pathologies. During tumor development or viral infection NKR-P1C may change expression patterns potentially affecting immune surveillance and responses. It works in tandem with proteins like perforin and granzymes which are involved in inducing cell death in target cells making it a significant player in immune responses to malignancies and persistent viruses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - Alexa Fluor® 488 Rabbit monoclonal [EPR22990-12] to NKR-P1C (ab322225), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit monoclonal [EPR22990-12] to NKR-P1C (ab322225)

    Flow cytometric analysis of Mouse splenocytes cells labelling NKR-P1C with ab322225 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Cells are co-stained with CD19 conjugated to PE/CY7.

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 488 Rabbit monoclonal [EPR22990-12] to NKR-P1C (ab322225), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Rabbit monoclonal [EPR22990-12] to NKR-P1C (ab322225)

    Flow cytometric analysis of Mouse splenocytes cells labelling NKR-P1C with ab322225 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Cells are co-stained with CD49b conjugated to PE.

    Gated on viable cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com