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AB200976

Alexa Fluor® 488 Anti-NRF1 antibody [EPR5554(N)]

5

(1 Review)

|

(3 Publications)

Rabbit Recombinant Monoclonal NRF1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

Nuclear respiratory factor 1, NRF-1, Alpha palindromic-binding protein, Alpha-pal, NRF1

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-NRF1 antibody [EPR5554(N)] (AB200976)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-NRF1 antibody [EPR5554(N)] (AB200976)

ab200976 staining NRF1 in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200976 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2μg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-NRF1 antibody [EPR5554(N)]

  • Unconjugated

    Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade

  • Carrier free

    Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5554(N)

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB268214

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Primary Antibodies

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Primary Antibodies

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Complementary Products

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

AB192890

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Western blot - Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB192890)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The nuclear respiratory factor 1 (NRF1) also known as Alpha-Pal is a transcription factor with an approximate molecular weight of 68 kDa. It plays an important role in the regulation of genes required for mitochondrial DNA transcription and replication. Researchers find NRF1 expression in various tissues including muscle brain and liver. This protein functions mechanistically by binding to specific promoter regions of target genes to control their expression making it essential for maintaining cellular energy metabolism.
Biological function summary

NRF1 influences the expression of genes involved in the mitochondrial function and is part of the transcriptional complex. This complex includes the NRF2 protein where NRF1 partners with co-activators to modulate gene transcription. NRF1 promotes the expression of components essential for mitochondrial biogenesis and cellular respiration which are vital processes for energy production in cells. Therefore NRF1 contributes to maintaining cellular energy homeostasis and overall cellular health.

Pathways

NRF1 integrates into critical regulatory pathways for mitochondrial function and metabolism. NRF1 links closely to the PGC-1α pathway which is instrumental in the regulation of energy metabolism and mitochondrial biogenesis. Additionally NRF1 works in conjunction with NRF2 to regulate oxidative stress responses. These interactions ensure a coordinated cellular response to energy demands and help protect the cell from oxidative damage.

NRF1 associates with neurodegenerative diseases and metabolic syndromes. Altered NRF1 regulation can impact diseases such as Alzheimer's disease where it may contribute to neurodegeneration through impaired mitochondrial function. Moreover the dysfunction of NRF1 might relate to metabolic disorders like diabetes as energy metabolism and mitochondrial health are disrupted. NRF1 connects with proteins like SIRT1 in these contexts further implicating NRF1 in these pathological states through its role in energy homeostasis and stress response.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
See full target information NRF1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 5:102754 PubMed38096060

2023

Protocol for cross-platform characterization of human and murine extracellular vesicles and particles.

Applications

Unspecified application

Species

Unspecified reactive species

Linda Bojmar,Han Sang Kim,Kei Sugiura,Søren Heissel,Serena Lucotti,Michele Cioffi,Kofi Ennu Johnson,Leona Cohen-Gould,Haiying Zhang,Henrik Molina,Irina R Matei,David Lyden,Ayuko Hoshino

Lipids in health and disease 22:33 PubMed36882837

2023

Exercise activates Sirt1-mediated Drp1 acetylation and inhibits hepatocyte apoptosis to improve nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Zongqiang Hu,Hongyu Zhang,Yiting Wang,Boyi Li,Kaiyu Liu,Jianghua Ran,Li Li

Cell death & disease 11:1030 PubMed33268822

2020

Telomeric injury by KML001 in human T cells induces mitochondrial dysfunction through the p53-PGC-1α pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Madison Schank,Juan Zhao,Ling Wang,Zhengke Li,Dechao Cao,Lam Nhat Nguyen,Xindi Dang,Sushant Khanal,Lam Ngoc Thao Nguyen,Bal Krishna Chand Thakuri,Stella C Ogbu,Zeyuan Lu,Jinyu Zhang,Xiao Y Wu,Zheng D Morrison,Mohamed El Gazzar,Shunbin Ning,Jonathan P Moorman,Zhi Q Yao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com