AB154028

Alexa Fluor® 488 Anti-Nucleolin antibody [364-5]

Name: Anti-Nucleolin Alexa Fluor® 488 antibody [364-5] (ab154028) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB154028
Price: 545 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal Nucleolin antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Nucleolin, Protein C23, NCL

3 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nucleolin antibody [364-5] (AB154028)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nucleolin antibody [364-5] (AB154028)

Immunocytochemistry with HeLa cells were stained with Anti-Nucleolin-488 antibody (0.2 µg/mL, ab154028) in green, Anti-GAPDH (1.0 µg/mL, ab110305) as red, and DAPI (1/10,000) in blue, as a nuclear stain. Secondary antibody used was goat anti-mouse IgG1 Alexa-594 at 1/1000 dilution. A) HeLa cells 40x. B) HeLa cells 100x.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nucleolin antibody [364-5] (AB154028)

Immunocytochemistry with HDFn cells were stained with Anti-Nucleolin-488 antibody (0.2 µg/mL, ab154028) in green, Anti-GAPDH (1.0 µg/mL, ab110305) as red, and DAPI in blue, as a nuclear stain. Secondary antibody used was goat anti-mouse IgG1 Alexa-594 at 1/1000 dilution. A) HDFn 40x. B) HDFn 100x.

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Nucleolin antibody [364-5] (AB154028)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Nucleolin antibody [364-5] (AB154028)

Flow Cytometry for Anti-Nucleolin-488 antibody (ab154028) stained HeLa cells. Flow cytometry results of unstained cells (black) and 1.0 µg/mL Nucleolin-488 (red) stained cells.

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Nucleolin antibody [364-5]
Carrier free

Anti-Nucleolin antibody [364-5] - BSA and Azide free
Unconjugated

Anti-Nucleolin antibody [364-5]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

364-5

Isotype

IgG1

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1 µg/mL", "FlowCytIntra-species-notes": "<a href='/en-us/products/unavailable/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-alexa-fluorreg-488-ab171463'>ab171463</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.2 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1 µg/mL", "FlowCytIntra-species-notes": "<a href='/en-us/products/unavailable/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-alexa-fluorreg-488-ab171463'>ab171463</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "0.2 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1 µg/mL", "FlowCytIntra-species-notes": "<a href='/en-us/products/unavailable/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-alexa-fluorreg-488-ab171463'>ab171463</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "0.2 µg/mL", "ICCIF-species-notes": "" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

ab154028 is purified by biochemical fractionation. Near homogeneity as judged by SDS-PAGE.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleolin also known as C23 is a multifunctional protein mainly found in the nucleolus with a molecular weight of approximately 100 kDa. It plays a mechanical role in ribosomal RNA (rRNA) synthesis and ribosome biogenesis. Nucleolin is widely expressed in proliferating cells including HeLa culture and shows higher expression in cancer cells compared to normal cells. The nucleolin protein is involved in various nucleolar processes like the modulation of chromatin structure and nucleocytoplasmic transport.

Biological function summary

The nucleolin molecular weight protein contributes to regulating cellular processes such as chromatin remodeling rRNA transcription and mRNA stability. Nucleolin often forms complexes with various other proteins and nucleic acids enhancing its functionality within the cell nucleus. Its participation in processes like DNA replication and cell proliferation shows the central role nucleolin plays at the cellular level. It influences cellular responses by binding directly to specific RNA or DNA sequences.

Pathways

The nucleolin protein engages in major cellular pathways such as the RNA processing and p53 signaling pathways. Through the RNA processing pathway it interacts with proteins like fibrillarin to assist in rRNA maturation. Moreover within the p53 signaling pathway nucleolin regulates the activity of the tumor suppressor protein p53 impacting cell cycle progression and apoptosis. These interactions highlight nucleolin's role in maintaining cellular homeostasis and response to stress.

The nucleolin protein often shows altered expression in cancers and cardiovascular diseases. In cancer nucleolin's high expression levels correlate with tumor progression and metastasis making it a potential target for cancer therapies. In cardiovascular diseases nucleolin interacts with proteins like VEGF to influence angiogenesis which affects disease progression. Understanding nucleolin's involvement in these diseases provides insight into potential therapeutic targets and interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

See full target information NCL

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70323 PubMed39812615

2025

A noncanonical-GPRC5A signaling regulates keratinocyte adhesion and migration by nuclear translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Chanteloube,Choua Ya,Gabrielle Le Provost,Aurore Berthier,Cindy Dieryckx,Sandrine Vadon-Le Goff,Florence Nadal,Bérengère Fromy,Romain Debret

Nucleic acids research 47:3086-3100 PubMed30624716

2019

Nuclear processing of nascent transcripts determines synthesis of full-length proteins and antigenic peptides.

Applications

Unspecified application

Species

Unspecified reactive species

Rodrigo Prado Martins,Laurence Malbert-Colas,María José Lista,Chrysoula Daskalogianni,Sebastien Apcher,Marika Pla,Sarah Findakly,Marc Blondel,Robin Fåhraeus

Journal of cellular biochemistry 120:4804-4812 PubMed30390333

2018

Optimization of prostate cancer cell detection using multiplex tyramide signal amplification.

Applications

Unspecified application

Species

Unspecified reactive species

Sounak Roy,Haley D Axelrod,Kenneth C Valkenburg,Sarah Amend,Kenneth J Pienta

Molecular cancer research : MCR 17:356-369 PubMed30291220

2018

AXL Is a Putative Tumor Suppressor and Dormancy Regulator in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Haley D Axelrod,Kenneth C Valkenburg,Sarah R Amend,Jessica L Hicks,Princy Parsana,Gonzalo Torga,Angelo M DeMarzo,Kenneth J Pienta

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-Nucleolin antibody [364-5]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nucleolin antibody [364-5] (AB154028)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nucleolin antibody [364-5] (AB154028)

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Nucleolin antibody [364-5] (AB154028)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise