Alexa Fluor® 488 Anti-Nucleolin antibody [364-5]

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Nucleolin also known as C23 is a multifunctional protein mainly found in the nucleolus with a molecular weight of approximately 100 kDa. It plays a mechanical role in ribosomal RNA (rRNA) synthesis and ribosome biogenesis. Nucleolin is widely expressed in proliferating cells including HeLa culture and shows higher expression in cancer cells compared to normal cells. The nucleolin protein is involved in various nucleolar processes like the modulation of chromatin structure and nucleocytoplasmic transport.

Biological function summary

The nucleolin molecular weight protein contributes to regulating cellular processes such as chromatin remodeling rRNA transcription and mRNA stability. Nucleolin often forms complexes with various other proteins and nucleic acids enhancing its functionality within the cell nucleus. Its participation in processes like DNA replication and cell proliferation shows the central role nucleolin plays at the cellular level. It influences cellular responses by binding directly to specific RNA or DNA sequences.

Pathways

The nucleolin protein engages in major cellular pathways such as the RNA processing and p53 signaling pathways. Through the RNA processing pathway it interacts with proteins like fibrillarin to assist in rRNA maturation. Moreover within the p53 signaling pathway nucleolin regulates the activity of the tumor suppressor protein p53 impacting cell cycle progression and apoptosis. These interactions highlight nucleolin's role in maintaining cellular homeostasis and response to stress.

Associated diseases and disorders

The nucleolin protein often shows altered expression in cancers and cardiovascular diseases. In cancer nucleolin's high expression levels correlate with tumor progression and metastasis making it a potential target for cancer therapies. In cardiovascular diseases nucleolin interacts with proteins like VEGF to influence angiogenesis which affects disease progression. Understanding nucleolin's involvement in these diseases provides insight into potential therapeutic targets and interventions.